TISSUE ENGINEERING FOR PERIODONTIUM REPAIR

牙周修复组织工程

• 批准号: 6193172
• 负责人: Margarita Zeichner-David
• 金额: $ 26.47万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6193172
• 关键词: TISSUE; ENGINEERING; PERIODONTIUM; REPAIR

DESCRIPTION: (verbatim) Tissue Engineering is defined as the science developing techniques for fabrication of new tissues for replacement and regeneration of lost tissues based on principles of cell and developmental biology. One of the major problems in aging, periodontal disease, tooth implants, transplantation, or replantation remains the repair of the periodontium and the regeneration of periodontal tissues such as cementum, periodontal ligament and alveolar bone. In general, to achieve complete repair and regeneration, it is necessary to recapitulate the process of embryogenesis and morphogenesis involved in the formation of that tissue. In the case of the periodontium, complete periodontal repair entails de novo cementogenesis, osteogenesis, and periodontal ligament fibers. Current strategies for periodontal repair have been directed at enhancing alveolar bone and periodontal ligament regeneration and have the problem that do not completely restore the architecture of the periodontium. The long-term objective of this project is to achieve complete periodontium regeneration by focusing on the cementum layer using tissue engineering to induce de novo cementogenesis. We propose to test the hypothesis that HERS cells maintained in vitro can re-colonize cementum denuded roots and recreate the process of cementogenesis. Furthermore, the cementum-like mineralized extracellular matrix deposited by these cells in vitro (or some of its components) will induce complete periodontal regeneration. We propose to test this hypothesis by using an Immortomouse-derived HERS cell line established in our laboratory to re-colonize root dentine in vivo. In similar experiments, the extracellular matrix produced by these cells and some of its isolated components (mainly enamel proteins) will also be tested for their inductive and reparative capability. The results obtained in this study can then be applied, alone or in conjunction with known techniques for bone regeneration, for the treatment of periodontal disease and to improve the long-term success of implants. The data generated in this study will also provide information related to the regulation of root formation and possible ways to improve the diagnosis, treatment, and prognosis of tooth loss due to aging or periodontal diseases.

描述：（逐字）组织工程被定义为科学发展 用于替代和再生的新组织制造的技术 基于细胞和发育生物学原理的损失组织。中的一个 衰老，牙周疾病，牙齿植入物，移植的主要问题， 或补给仍然是牙周修复和再生 牙周组织，例如牙骨质，牙周韧带和肺泡骨。 通常，要实现完整的维修和再生，有必要 概括了涉及的胚胎发生和形态发生的过程 该组织的形成。如果是牙周，请完成牙周 维修需要从头胶结生成，成骨和牙周韧带 纤维。当前的牙周维修策略已针对 增强牙槽骨和牙周韧带再生，并具有 无法完全恢复牙周结构的问题。 该项目的长期目标是实现完整的牙周 通过使用组织工程专注于牙骨质层来再生 诱导从头胶结发生。我们建议检验她的假设 维持体外的细胞可以重新殖民化胶质的根并重现 胶结发生过程。此外，牙骨质状矿化 这些细胞在体外沉积的细胞外基质（或其中一些 组件）将引起完整的牙周再生。我们建议测试 通过使用不朽的衍生的HERS系列，该假设在 我们在体内重新殖民牙本质的实验室。在类似的实验中， 这些细胞产生的细胞外基质及其某些分离 成分（主要是搪瓷蛋白）也将用于其感应性和 修复能力。然后可以应用本研究中获得的结果， 单独或与已知的骨再生技术结合 治疗牙周疾病并改善 植入物。本研究产生的数据还将提供信息 与根系的调节以及改善的可能方法有关 诊断，治疗和预后因衰老或牙周造成的牙齿脱落 疾病。