The application of modern Synthetic Biology techniques to address the production of nutritional and industrial speciality chemicals

应用现代合成生物学技术来解决营养和工业特种化学品的生产

• 批准号: 2289306
• 金额: --
• 依托单位: Royal Holloway University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2289306
• 关键词: application; modern; Synthetic; Biology; techniques

Isoprenoids, also known as terpenoids represent the largest and oldest class of natural products documented. In crop plants intense efforts have been made to increase nutritional isoprenoids such as carotenoids (b-carotene provitamin A) and tocopherols (vitamin E) as a means of nutritional enhancement. Commercially isoprenoids are used in cosmetics and fragrances, and as colorants and nutritional supplements in foods and feeds. The present production platforms are either plants and/or microorganisms. Biosynthetically all isoprenoids are related via a common five carbon building block (isopentenyl pyrophosphate; IPP). Different isoprenoid classes can utilise the same isoprenoid precursors, which are preferentially used or channeled at different developmental stages. Through the creation of enzyme fusions a means of altering/optimising metabolite challenging can been achieved. These fusions which utilise flexible peptide linkers have the potential to optimise the flow of precursors from one class of isoprenoid into the desired products. In effect create an artificial metabolon. Proof of concept work has proven the potential of the system both in terms of enzymatic function and high throughput screening. In the proposed project fusions between isoprenoid enzymes metabolising geranylgeranyl (C20) or farnesyl (C15) diphosphate will be created and tested in plants and yeasts.

类异型，也称为萜类化合物是所记录的最大，最古老的天然产品。在作物植物中，已经采取了强烈的努力，以增加营养的类异on-（例如胡萝卜素（B-胡萝卜素Propitamin A）和生育酚（维生素E），作为营养增强的一种手段。商业上的类异戊二烯用于化妆品和香料中，作为食物和饲料中的着色剂和营养补充剂。当前的生产平台是植物和/或微生物。生物合格从生物合成中，所有类吸引者都是通过常见的五碳构建块（异戊烯基焦磷酸； IPP）相关的。不同的类异丙类别可以利用相同的类异丙素前体，这些原子体在不同的发育阶段优先使用或引导。通过创建酶融合，可以实现改变/优化代谢物挑战的方法。这些利用柔性肽接头的融合物具有优化从一类类异丙素的前体流入所需产品的可能性。实际上，创建了人造代谢龙。概念验证工作证明了该系统在酶促功能和高吞吐量筛选方面的潜力。在拟议的项目融合中，将在植物和酵母菌中创建和测试异丙酶代谢的黄烷基（C20）或Farnesyl（C15）二磷酸盐（C15）。