Genetic Control of Segmentation

分割的遗传控制

• 批准号: 6615669
• 负责人: SUSAN J. BROWN
• 金额: $ 29.05万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6615669
• 关键词: Coleoptera; body regions; cytogenetics; developmental genetics; early embryonic stage; embryogenesis; gene expression; gene interaction; gene mutation; genetic library; genetic manipulation; genetic mapping; genetic regulation; homeobox genes; in situ hybridization; molecular cloning; polymerase chain reaction; protein structure function; regulatory gene; southern blotting; species difference; western blottings

DESCRIPTION (provided by applicant): Through comparative studies between distantly related animals, it has become apparent that genes and regulatory networks functioning during embryonic growth are highly conserved. This has led to the hypothesis that evolutionary changes in morphology can be traced to alterations in these regulatory modules. Studies to address this hypothesis have focused on insects, which display different modes of segmentation. Most approaches rely on cloning and expression analysis of orthologs of well-characterized Drosophila genes. However, most insects do not offer facile approaches to examine the functional significance of conserved gene expression patterns, or to test observed differences. Further, these comparisons are limited to the analysis of mechanisms discovered in flies, and do not offer the possibility of identifying genes important to segmentation in species other than flies. Our studies in Tribolium overcome these limitations, since Tribolium offers the possibility of genetic manipulation in addition to its facility for developmental and molecular studies. Moreover, the recent advances in RNA interference and germline transformation place Tribolium in the forefront of comparative model systems. We have discovered that depletion of certain pair-rule gene mRNAs by RNAi blocks segmentation and morphogenesis in Tribolium, results not predicted by the Drosophila paradigm. To understand the molecular interaction underlying these novel phenotypes we will examine the effects of Tceve and Tcrun mRNA depletion on the expression of other segmentation and homeotic genes. Analysis of the regulatory regions associated with these genes and ectopic expression of transgenes will complement the RNAi studies. To discover other genes important to segmentation in Tribolium we will execute a transposon-tagging mutagenesis scheme and characterize relevant mutants. Our research provides a unique opportunity to elucidate the genetic mechanisms underlying the regulation of the process of progressive segmentation in a cellular environment.

描述（由申请人提供）：通过 遥远相关的动物，很明显基因和调节性 胚胎生长期间的网络功能是高度保守的。这已经引起了 假设形态的进化变化可以追溯到 这些法规模块的改变。解决这一假设的研究 专注于昆虫，这些昆虫显示出不同的分割模式。最多 方法依赖于克隆和表达分析的直系同源 特征良好的果蝇基因。但是，大多数昆虫不提供便利 检查保守基因表达的功能意义的方法 模式，或测试观察到的差异。此外，这些比较是 仅限于在蝇中发现的机制的分析，并且不提供 鉴定对种群分割重要的基因的可能性 比苍蝇。我们在Tribolium的研究克服了这些局限性，因为 Tribolium还提供了基因操纵的可能性 发育和分子研究的设施。而且，最近的进步 在RNA干扰和种系转化中 比较模型系统的最前沿。 我们发现RNAi对某些成对规则基因mRNA的耗竭 跨越的块分割和形态发生，结果不预测 果蝇范式。了解基础的分子相互作用 这些新型的表型我们将检查TCEVE和TCRUN mRNA的影响 其他分割和同源基因的表达耗竭。分析 与这些基因相关的调节区域和异位表达 转基因将补充RNAi研究。发现其他重要的基因 要在Tribolium中进行分割，我们将执行转座子诱变诱变 方案并表征相关突变体。我们的研究提供了独特的 阐明调节基础的遗传机制的机会 在细胞环境中进行性分割的过程。