Oxidative Stress, Antioxidant Status & Pregnancy Outcome

氧化应激、抗氧化状态

• 批准号: 6777969
• 负责人: Theresa O. Scholl
• 金额: $ 4.67万
• 依托单位: UNIV OF MED/DENT NJ-SCH OSTEOPATHIC MED
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-11-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6777969
• 关键词: Oxidative; Stress; Antioxidant; Status; Pregnancy

DESCRIPTION (provided by applicant): We hypothesize that the risk of low birth weight and other poor pregnancy outcomes in low income and minority women is associated with increased oxidative stress. The parent study tests this hypothesis in 1,200 low-income and minority pregnant women from Camden, NJ by measuring the influence of urinary excretion of 8-iso-PGF-2alpha and 8-oxo-7,8 dihydro-2 deoxyguanosine (8-OH-dG) excretion, in the maternal-fetal unit on low birth weight and other poor pregnancy outcomes. 8-OH-dG is a surrogate for the assessment of oxidative damage to the DNA. The objective of this supplemental project is to complete the project by answering questions not specifically addressed in the original application. Specific aims are as follows: To measure the 8-OH-dG content of fetal and maternal lymphocyte DNA and to correlate them with urinary 8-OH-dG at week 28. To determine the importance of fetal lymphocyte 8-OH-dG for low birth weight, fetal growth restriction and other poor outcomes. To collect and stabilize cord blood mRNA for future research. To continue enrolling women into the cohort, monitoring them during pregnancy and collecting cord blood at delivery.

描述（由申请人提供）：我们假设，低出生体重和其他低收入和少数族裔妇女的妊娠结局的风险与增加的氧化压力有关。父母研究通过测量8-ISO-PGF-2ALPHA的尿液排泄的影响和8-OXO-7,8 Dihydro-2 DeoxyGuanosonosine（8-OH-DG）的尿位对食物 - 五型甲壳虫的排泄量，在较低的生育体重和其他不良的生育体重。 8-OH-DG是评估DNA氧化损伤的替代物。该补充项目的目的是通过回答原始应用程序中未具体解决的问题来完成该项目。具体目的如下：测量胎儿和母体淋巴细胞DNA的8-OH-DG含量，并在第28周将它们与尿液8-OH-DG相关联。确定胎儿淋巴细胞8-OH-DG对于低出生体重，胎儿生长限制和其他较差的效果的重要性。 收集和稳定脐带血mRNA以进行未来的研究。 为了继续让妇女加入队列，在怀孕期间监测她们并在分娩时收集脐带血。