Optical imaging of neural activity in auditory system

听觉系统神经活动的光学成像

• 批准号: 6614294
• 负责人: James A Kaltenbach
• 金额: $ 13.06万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6614294
• 关键词: auditory nuclei; bioimaging /biomedical imaging; electrophysiology; hamsters; imaging /visualization /scanning; neural conduction; neural information processing; neuroimaging; neurons; neurophysiology; optics; sound

DESCRIPTION (provided by applicant): This project will examine the potential of optical imaging technology for the investigation of functional activity on the surface of the dorsal cochlear nucleus (DCN). This technology has recently come of age as a result of a new generation of optical imaging cameras characterized by ultra high spatial and temporal resolution and the introduction of new, non-toxic voltage sensitive dyes. When these new tools are used in combination, optical imaging provides a powerful approach to mapping brain activity in real time. In the proposed project we will employ a state-of-the-art optical imaging system to study the spatio-temporal dynamics of stimulus-driven and spontaneous activity on the surface of the DCN. First, we will obtain real time image sequences of DCN responses to acoustic stimuli that change in frequency and intensity. The purpose of this experiment is to test the feasibility of optical imaging to examine the spatial and temporal dynamics of information flow within and across the tonotopic array of the DCN. Second, we will use optical imaging to map and quantify increases in spontaneous activity that can be induced in the DCN by intense sound exposure. The goal will be to determine the spatial patterns of this hyperactivity and its temporal behavior. Third, we will image the spatio-temporal patterns of activity that occur on the DCN surface in response to parallel fiber stimulation. The image sequences will be acquired during and after electrical stimulation of parallel fibers at a number of sites across the DCN surface. The goal is to map the path of activation as it flows along the parallel fibers to its target neurons. The significance of these experiments is that they will yield information regarding the dynamics of signal processing and reveal new aspects of DCN circuitry that have been difficult to study electrophysiologically.

描述（由申请人提供）：该项目将检查光学成像技术对背侧耳蜗核表面功能活性研究（DCN）的潜力。由于新一代的光学成像摄像头，该技术最近已经成熟，其特征是超高空间和时间分辨率以及引入新的无毒电压敏感染料。当这些新工具结合使用时，光学成像为实时绘制大脑活动提供了强大的方法。在拟议的项目中，我们将采用最先进的光学成像系统来研究DCN表面上刺激驱动和自发活动的时空动力学。首先，我们将获得对频率和强度变化的原声刺激反应的实时图像序列。该实验的目的是测试光学成像的可行性，以检查DCN内部和整个吨位阵列中信息流的空间和时间动力学。其次，我们将使用光学成像来绘制和量化自发活动的增加，这可以通过强烈的声音暴露在DCN中引起。目标是确定这种多动症的空间模式及其时间行为。第三，我们将图像响应平行纤维刺激的DCN表面上发生的活性的时空模式。图像序列将在跨DCN表面的许多位点进行电刺激期间和之后获取。目的是将激活路径沿平行纤维流向其靶神经元时。这些实验的意义在于它们将产生有关信号处理动力学的信息，并揭示DCN电路的新方面，这些方面很难在电生理上研究。