HUMAN REPLICATION PROTEIN COMPLEXED W/ SINGLE STRANDED DNA

与单链 DNA 复合的人类复制蛋白

• 批准号: 6437536
• 负责人: ALED M EDWARDS
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437536
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

Replication protein A (RPA) is a key component of the DNA replication and repair machineries. RPA is a single stranded DNA binding protein that interacts with DNA replication origin binding proteins, DNA polymerases, transcription activator proteins and DNA repair factors, including those that are mutated in several forms of human cancer. RPA is a heterotrimer; the DNA binding domain has been localized to the largest subunit. We have used limited proteolysis and deletion analysis to identify the minimal single strand DNA binding domain. We have crystallized this domain together with ssDNA. We are pursuing heavy atom searches on our unit and have also prepared selenomethionine-labeled protein for co-crystallization.

复制蛋白 A (RPA) 是 DNA 的关键组成部分 复制和修复机器。 RPA是单链DNA 与 DNA 复制起点结合相互作用的结合蛋白 蛋白质、DNA 聚合酶、转录激活蛋白和 DNA 修复因子，包括那些以多种形式突变的因子 人类癌症。 RPA 是异源三聚体； DNA 结合域已 定位于最大的亚基。 我们使用了有限的蛋白水解 和缺失分析以确定最小单链 DNA 结合域。 我们已经将该结构域与 ssDNA 一起结晶。 我们正在对我们的装置进行重原子搜索，并已做好准备 用于共结晶的硒代蛋氨酸标记的蛋白质。