Consomic Strains Between C57BL/6 and PWD

C57BL/6 和 PWD 之间的Consomic 菌株

• 批准号: 6764917
• 负责人: Beverly J Paigen
• 金额: $ 36.62万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-11 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6764917
• 关键词: aging; atherosclerosis; cholelithiasis; cryopreservation; diabetes mellitus; fertility; functional /structural genomics; genetic mapping; genetic models; genetic strain; hearing; hematology; high throughput technology; kidney function; laboratory mouse; learning; lung; memory; model design /development; molecular cloning; obesity; osteoporosis; phenotype; quantitative trait loci; respiratory function

DESCRIPTION (provided by applicant): We propose to develop a new and powerful combined genetic and information research tool to further enhance the utility of the mouse as a model organism for understanding human biology. This is a set of 22C57BL/6J-Chr#PWD consomic (chromosome substitution) strains, in which each strain carries a single different chromosome from the PWD inbred strain on a C57BL/6J (B6) genetic background. Recent observations indicate that much of the genetic variation among inbred mouse strains derives from the contributions of the two subspecies Mus musculus domesticus and M. m. musculus suggesting that introgressing the chromosomes of one of these subspecies into the other would capture most variation among common strains, providing a "universal set of consomics". The set of consomic strains that is nearing completion in the Czech Republic comes close to this ideal because the chromosomes of PWD, a pure M. m. musculus strain, are being introgressed into the B6 background, which is 80% M. m. domesticus. We will import these strains into The Jackson Laboratory, make them widely available, and characterize them for a range of physiologically important phenotypes and deposit the resulting information in the existing Mouse Phenome Database. The phenotypes include growth and fertility, sleep disorders, lung function, kidney function, obesity, diabetes, atherosclerosis, gallstones, hematology, osteoporosis, neurological function, immunologic function, hearing, sight, learning, memory, and aging. Phenotyping this consomic set will immediately identify chromosomes that contain genes affecting specific traits of biomedical interest. These data and this set of mice will provide a variety of mouse models to the scientific community that can be used to further explore the genetic basis of complex diseases and phenotypes. The post-genomic challenge of linking genotype and phenotype will require broad-scale, systems-wide approaches and infrastructure. We suggest that the availability of these consomic mice characterized for many phenotypes will help to meet that challenge.

描述（由申请人提供）：我们建议开发一种新的和强大的遗传和信息研究工具，以进一步增强小鼠作为模型生物的效用，以理解人类生物学。这是一组22C57BL/6J-CHR＃PWD CONSOMIC（染色体取代）菌株，其中每种菌株在C57BL/6J（B6）遗传背景上携带与PWD Inbred菌株的单个不同染色体。最近的观察结果表明，近交小鼠菌株之间的许多遗传变异源自两个亚种的贡献，含有家族的musculus ussculus and M. m。肌肉表明，将其中一个亚种的一个染色体浸入另一个，将捕获普通菌株之间的大多数变化，从而提供“通用的综合”。捷克共和国即将完成的一组综合菌株接近这一理想，因为PWD的染色体是纯M. m。肌肉菌株正在渗入B6背景，即80％m。家里。我们将将这些菌株导入杰克逊实验室，使其广泛使用，并将其特征为一系列重要的生理重要表型，并将所得信息存储在现有的鼠标现象数据库中。表型包括生长和生育能力，睡眠障碍，肺功能，肾功能，肥胖，糖尿病，动脉粥样硬化，胆结石，血液学，骨质疏松症，神经功能功能，免疫功能，听力，视力，学习，学习，记忆，记忆和年龄。表型这个杂质集将立即识别包含影响生物医学兴趣特定特征的基因的染色体。这些数据和这组小鼠将为科学界提供各种小鼠模型，这些模型可用于进一步探索复杂疾病和表型的遗传基础。将基因型和表型联系起来的基因组挑战将需要广泛的，全系统的方法和基础设施。我们建议，这些为许多表型特征的这些综合小鼠的可用性将有助于应对这一挑战。