Tiling Path Microarray Re-amplifable Resource Drosophila

平铺路径微阵列可再扩增果蝇资源

• 批准号: 6778006
• 负责人: KEVIN P. WHITE
• 金额: $ 107.51万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-16 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6778006
• 关键词: Drosophilidae; RNA interference; biomedical resource; data management; experimental designs; genome; high throughput technology; microarray technology; molecular biology information system; nucleic acid amplification techniques; nucleic acid repetitive sequence; polymerase chain reaction; technology /technique development

DESCRIPTION (provided by applicant): We propose to develop a re-amplifiable tiling path resource for the entire euchromatic Drosophila genome. This resource will be used for the production of genomic DNA microarrays to be distributed to the community at cost of printing. These arrays can be used for DNA-protein interaction mapping, comprehensive expression profiling, replication origin mapping, comparative genomic hybridization, physical mapping of deletions, and other applications. Protocols for use with this resource will be developed and disseminated, and a database and data management service will be established for use by users of the whole genome tiling path microarrays. This resource will be distributed to a wide community of researchers through the Drosophila Genomics Resource Center. Additionally, a strategy is proposed to use a newly developed, well-addressable system of re-amplifiable tags. The entire genome in the form of lkb PCR products will be distributable to individual labs in four 384 well plates. A researcher will be able to amplify any or all of the genome tiling fragments using pairs of 20 unique primers. This feature of the proposed resource would enable comprehensive RNAi screens, rapid cloning of regulatory sequences, and potentially many other applications.

描述（由申请人提供）：我们建议为整个常染色质果蝇基因组开发可重新扩增的平铺路径资源。该资源将用于生产基因组 DNA 微阵列，并以打印成本分发给社区。这些阵列可用于 DNA-蛋白质相互作用图谱、综合表达谱分析、复制起点图谱、比较基因组杂交、删除的物理图谱和其他应用。将开发和传播使用该资源的协议，并将建立数据库和数据管理服务，供全基因组平铺路径微阵列的用户使用。该资源将通过果蝇基因组学资源中心分发给广泛的研究人员群体。 此外，还提出了一种策略，即使用新开发的、可良好寻址的可重放大标签系统。 lkb PCR 产物形式的整个基因组将分布在四个 384 孔板中的各个实验室。研究人员将能够使用 20 个独特引物对扩增任何或全部基因组拼接片段。所提议资源的这一特征将实现全面的 RNAi 筛选、调节序列的快速克隆以及潜在的许多其他应用。