Laser Catheter for Dethrombosis of Arterial Occlusion

激光导管用于动脉闭塞除血栓

• 批准号: 6765177
• 负责人: BRANT D WATSON
• 金额: $ 17.52万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6765177
• 关键词: artery occlusion; biomedical equipment development; carotid artery; clinical biomedical equipment; erythrocytes; fibrin; fluoroscopy; histology; laboratory rat; laser therapy; light microscopy; method development; nonhuman therapy evaluation; platelets; statistics /biometry; thrombosis; ultraviolet radiation

DESCRIPTION (provided by applicant): The chief goal of this proposal is construct a catheter-based interventional device containing an ultraviolet (UV) light-conducting glass optical fiber, which will be used to remove, from a common carotid artery (CCA) of a rat, an occlusive blood clot (thrombus) and to demonstrate, in particular, the effectiveness of this device against thrombi containing platelets only, or containing fibrin, red blood cells and platelets. The basis of this device's function is our newly patented (U.S. No. 6,539,944) process of ultraviolet (UV) laser-facilitated dethrombosis, defined as the dissolution of a platelet aggregate into individual platelets without the use of a thrombolytic agent. Thrombus dissociation is initiated by endoscopic UV laser irradiation of the interior surface of the arterial segment just proximal to the thrombus. This facilitates smooth muscle relaxation and thus dilation of the arterial segment, and most unusually, of the thrombus also. This allows for efficient penetration of blood into and throughout the thrombus and presages recanalization of the artery. UV laser irradiation proceeds sequentially through the thrombus, as one segment after another is recanalized. We will first construct a prototype single-lumen catheter, which can admit, via separate channels, an optical fiber to conduct the UV laser light and also infusion of solutions, such as saline, X-ray contrast fluid, or drugs. We will then insert the device via a femoral artery under X-ray fluoroscopy, first to examine the effectiveness of this device to dilate the rat CCA, and then to use these experimental parameters to begin dethrombosis of pure platelet (PP) or platelet-fibrin-red cell (FPE) occlusive thrombi. It may be possible to observe the progress of dethrombosis by fluoroscopy even during UV irradiation, owing to only moderate absorption of the UV light by the X-ray contrast fluid. For the PP and FPE thrombi we will determine the time taken to recanalize the CCA after endoscopic UV treatment and compare these dissolution times to the corresponding times when UV treatment is applied in conjunction with a special thrombolytic (tPA) drug, reteplase, and to those times resulting from drug treatment itself. Finally, we will examine by histologic staining and microscopy the treated portion of the CCA for residual thrombotic material.

描述（由申请人提供）： 该提案的主要目的是构建一个基于导管的介入装置，其中包含紫外线光纤（UV）光纤维光纤，将用于从大鼠的常见颈动脉（CCA）中删除，闭塞性血凝块（血栓）（血栓）（血栓），尤其是该设备仅在含有液体的液体和平原的液体中，或者证明了该设备的有效性。该设备功能的基础是我们新的专利（美国第6,539,944号）紫外线（UV）激光辅助术的过程，定义为无需使用溶栓剂而将血小板聚集物溶解到单个血小板中。血栓解离是由动脉段内镜面紫外线激光辐射引发的，仅靠近血栓。这促进了平滑肌松弛，从而促进了动脉段的扩张，最异常的是血栓。这允许血液有效地渗透到整个血栓中，并预示动脉的重新定性。紫外线激光辐照通过血栓依次进行，因为一个接一个的一个段被重新加以重新加固。我们将首先构建一个原型的单光导管，该导管可以通过单独的通道接收光纤，以进行紫外线激光以及溶液的输注，例如盐水，X射线对比度或药物。然后，我们将在X射线荧光镜检查下通过股动脉插入设备，首先检查该设备扩张大鼠CCA的有效性，然后使用这些实验参数开始脱骨纯血小板（PP）或血小板 - 纤维蛋白 - 纤维蛋白 - 红细胞（FPE）咬合的链球菌。即使在紫外线照射期间，也可以通过荧光镜检查观察到链栓性的进展，因为仅通过X射线对比液对紫外线的中度吸收。对于PP和FPE血栓，我们将确定内窥镜紫外线治疗后重新合作CCA所花费的时间，并将这些溶解时间与相应的时间进行比较，当时与特殊的溶栓（TPA）药物，reteplase以及由药物治疗所产生的时代进行了紫外线处理。最后，我们将通过组织学染色和显微镜检查剩余的血栓形成材料的CCA处理部分。