Neurotransmission in developing cochlear ganglion cells

发育中的耳蜗神经节细胞的神经传递

• 批准号: 6694961
• 负责人: BRIAN P MANNING
• 金额: $ 4.16万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6694961
• 关键词: G protein; aminoglycoside antibiotics; auditory stimulus; calcium flux; chickens; cochlear nerve; confocal scanning microscopy; ear hair cell; ganglion cell; glutamate receptor; in situ hybridization; labyrinth; neural transmission; neuropharmacology; northern blottings; polymerase chain reaction; postdoctoral investigator; receptor expression; second messengers

DESCRIPTION (provided by applicant): Experiments are proposed to elucidate roles of glutamate receptors in the cochlear ganglion that are in addition to moment-by-moment transmission of auditory signals. The project arises from preliminary fura-2 calcium-imaging studies in which isolated chick ganglion cells show cyclic changes in intracellular calcium lasting for several minutes after injection of glutamate receptor agonists. This response is present only until embryonic day 16, raising the possibility that an alteration in glutamate receptor subtype underlies a shift in signal processing from primordial rhythmic bursting needed to develop and refine neural connections to the stochastic transmission of acoustic signal information needed for perception of sound. We will combine a pharmacological and molecular biological approach to characterize this transition in glutamate receptor expression.

描述（由申请人提供）：提出了实验，以阐明谷氨酸受体在耳蜗神经节中的作用，这些作用是在听觉信号的时刻传播中的补充。该项目是由初步的Fura-2钙成像研究引起的，其中孤立的鸡神经节细胞在注射谷氨酸受体激动剂后几分钟显示细胞内钙的循环变化。这种反应仅存在直到胚胎第16天才存在，这增加了谷氨酸受体亚型的改变是信号处理的转变，这是信号处理的转变，从原始的节奏爆发中转移，以发展和完善神经连接，再到声音感知所需的声学信号信息的随机传播。我们将结合一种药理和分子生物学方法，以表征谷氨酸受体表达中的这种过渡。