CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6583725
• 负责人: WILLIAM C. MERRICK
• 金额: $ 19.62万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6583725
• 关键词: AIDS; aminoacid analyzer; biomedical equipment purchase; biomedical facility; computer assisted sequence analysis; densitometry; fermentation; imaging /visualization /scanning; molecular biology; nucleic acid chemical synthesis; nucleic acid sequence; peptide chemical synthesis; protein sequence; surface plasmon resonance

The Molecular Biology Core Laboratory (MBCL) at Case Western Reserve University has been in operation for the past 10 years. The guiding principle has been to provide state-of-the-art scientific capabilities to the research community of Cleveland with special emphasis to the following supporting groups: CFAR, Cancer Center and kin Research Center. The MBCL is house in space renovated in 1990 specially for its use and offers the following services: protein sequencing, amino acid analysis, DNA synthesis, DNA sequencing, phosphorimaging, scanning laser densitometry, biofermentation, and fluorimaging. The instrumentation for the MBCL has been obtained through shared instrumentation grants (NIH, and VA), private foundations and center grants (CFAR). In the past five years, the MBCL laboratory has purchase about $500,000 worth of new instrumentation which in some instances has been to replace old instruments (DNA synthesizer, protein sequencers), but in most instances to provide new methodologies. In the current application, funds are requested to purchase a Pharmacia Biosensor BIAcore. At the present time, most of the costs of the MBCL are covered by user fees which are established for each services to reflect the real cost of that service (maintenance contract, effort of the MBCL staff, chemicals and upgrades of hardware or software as appropriate). The MBCL has been in operation for a considerable length of time and is used to establishing rates for user fees. These rates are reviewed at least semi-annually and in most instances, the rates are either maintained or reduced. It is the practice of the MBCL to have each service pay for itself and not have any one service require support through others. If the need for a specific service is ever deemed too minimal, the service may be considered for elimination. Key AIDS-related projects which have used the facility include: Comparing HIV 1 and RSV Proteases (Jonathan Leis, Ph.D CA52047); Analysis of amino acid sequences and glycosylation patterns of the V3 region of gp120 (Susan Eshleman, Ph.D. HD34348); Crystallization of HIV-1 reverse transcriptase/tRNAlys, complexes (Stuart F.J. Le Grice AI31147); Protective and diagnostic Mycobacterial antigens (R. Wallis, AI24298).

Case Western Reserve的分子生物学核心实验室（MBCL） 在过去的10年中，大学一直在运作。引导 原则是为最先进的科学能力提供 克利夫兰的研究社区特别强调以下 支持小组：CFAR，癌症中心和亲属研究中心。 MBCL 是在1990年经过翻新的太空中的房屋，专门用于使用，并提供 以下服务：蛋白质测序，氨基酸分析，DNA 合成，DNA测序，磷光影像，扫描激光密度法， 生物介质和荧光成像。 MBCL的仪器具有 已通过共享仪器赠款（NIH和VA）获得 基金会和中心补助（CFAR）。在过去的五年中，MBCL 实验室购买了约500,000美元的新仪器 在某些情况下，已经更换旧仪器（DNA合成器， 蛋白质序列），但在大多数情况下提供新方法。 在当前申请中，要求资金购买Pharmacia 生物传感器biacore。目前，MBCL的大部分费用是 由用户费用涵盖，以供每种服务反映 该服务的实际成本（维护合同，MBCL的努力 适当的工作人员，化学品和硬件或软件的升级）。这 MBCL已经运行了相当长的时间，并且被使用 建立用户费用费率。至少对这些费率进行审查 在半年度和大多数情况下，利率要么维护或 减少。 MBCL的实践是让每项服务付费 本身，没有任何服务需要通过他人的支持。如果是 特定服务的需求永远太少了，服务可能是 考虑消除。使用的关键辅助相关项目 设施包括：比较HIV 1和RSV蛋白酶（Jonathan Leis，博士学位 CA52047）;分析氨基酸序列和糖基化模式 GP120的V3地区（Susan Eshleman，博士HD34348）;结晶 HIV-1逆转录酶/Trnalys，复合物（Stuart F.J. Le Grice AI31147）;保护性和诊断性分枝杆菌抗原（R. Wallis， AI24298）。