CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6485956
• 负责人: WILLIAM C. MERRICK
• 金额: $ 19.62万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485956
• 关键词: AIDS; aminoacid analyzer; biomedical equipment purchase; biomedical facility; computer assisted sequence analysis; densitometry; fermentation; imaging /visualization /scanning; molecular biology; nucleic acid chemical synthesis; nucleic acid sequence; peptide chemical synthesis; protein sequence; surface plasmon resonance

The Molecular Biology Core Laboratory (MBCL) at Case Western Reserve University has been in operation for the past 10 years. The guiding principle has been to provide state-of-the-art scientific capabilities to the research community of Cleveland with special emphasis to the following supporting groups: CFAR, Cancer Center and kin Research Center. The MBCL is house in space renovated in 1990 specially for its use and offers the following services: protein sequencing, amino acid analysis, DNA synthesis, DNA sequencing, phosphorimaging, scanning laser densitometry, biofermentation, and fluorimaging. The instrumentation for the MBCL has been obtained through shared instrumentation grants (NIH, and VA), private foundations and center grants (CFAR). In the past five years, the MBCL laboratory has purchase about $500,000 worth of new instrumentation which in some instances has been to replace old instruments (DNA synthesizer, protein sequencers), but in most instances to provide new methodologies. In the current application, funds are requested to purchase a Pharmacia Biosensor BIAcore. At the present time, most of the costs of the MBCL are covered by user fees which are established for each services to reflect the real cost of that service (maintenance contract, effort of the MBCL staff, chemicals and upgrades of hardware or software as appropriate). The MBCL has been in operation for a considerable length of time and is used to establishing rates for user fees. These rates are reviewed at least semi-annually and in most instances, the rates are either maintained or reduced. It is the practice of the MBCL to have each service pay for itself and not have any one service require support through others. If the need for a specific service is ever deemed too minimal, the service may be considered for elimination. Key AIDS-related projects which have used the facility include: Comparing HIV 1 and RSV Proteases (Jonathan Leis, Ph.D CA52047); Analysis of amino acid sequences and glycosylation patterns of the V3 region of gp120 (Susan Eshleman, Ph.D. HD34348); Crystallization of HIV-1 reverse transcriptase/tRNAlys, complexes (Stuart F.J. Le Grice AI31147); Protective and diagnostic Mycobacterial antigens (R. Wallis, AI24298).

凯斯西储大学分子生物学核心实验室 (MBCL) 大学已经运营了10年。指导性的 原则是提供最先进的科学能力 克利夫兰研究界特别强调以下方面 支持团体：CFAR、癌症中心和亲属研究中心。 MBCL 是一座空间之家，于 1990 年专门为其使用进行了翻修，并提供 以下服务：蛋白质测序、氨基酸分析、DNA 合成、DNA 测序、磷光成像、扫描激光密度测定、 生物发酵和荧光成像。 MBCL 的仪器有 通过共享仪器拨款（NIH 和 VA）、私人 基金会和中心拨款（CFAR）。过去五年，MBCL 实验室购买了价值约 500,000 美元的新仪器 在某些情况下是为了更换旧仪器（DNA 合成器、 蛋白质测序仪），但在大多数情况下提供新的方法。 在当前的申请中，需要资金购买 Pharmacia 生物传感器 BIAcore。目前，MBCL 的大部分成本是 涵盖为每项服务设立的用户费用，以反映 该服务的实际成本（维护合同、MBCL 的努力 人员、化学品以及硬件或软件升级（视情况而定）。这 MBCL 已经运行了相当长的时间并被使用 制定使用费率。这些费率至少经过审查 每半年一次，在大多数情况下，费率要么维持不变，要么 减少。 MBCL的做法是让每项服务付费 本身并没有任何一项服务需要通过他人的支持。如果 对特定服务的需求被认为太小，该服务可能是 考虑消除。已使用的与艾滋病相关的重点项目 设施包括： 比较 HIV 1 和 RSV 蛋白酶（Jonathan Leis 博士 CA52047);氨基酸序列和糖基化模式分析 gp120 的 V3 区域（Susan Eshleman，博士 HD34348）；结晶 HIV-1 逆转录酶/tRNAlys，复合物（Stuart F.J. Le Grice AI31147);保护性和诊断性分枝杆菌抗原（R. Wallis， AI24298）。