Dissecting the role of root exudates in root density growth responses in plants

剖析根分泌物在植物根密度生长反应中的作用

基本信息

批准号：
2272100
负责人：
金额：
--
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2272100
关键词：
Dissecting role root exudates density

项目摘要

BackgroundPlants can detect the density of roots in the rhizosphere and use this information to determine the spatio-temporal allocation of root and shoot growth. The mechanisms by which plants detect and respond to root density remain unclear, but biochemical root exudates are likely to play an important role. Strigolactones are hormones that inhibit root and shoot system growth, but which are also exuded into the soil; they are therefore an excellent candidate signalling molecule for root density perception.Objectives1. Assess the role of strigolactones in root density perception and response.2. Identify novel exudates that act in plant communication in the rhizosphere.3. Understand the mechanisms by which plants respond to high root density.NoveltyAlthough plant communication in the rhizosphere is a long-recognized phenomenon, very little work has been undertaken to uncover the molecular signalling mechanisms that underlie it. This project thus represents a novel line of investigation into an important biological phenomenon. TimelinessInhibition of shoot growth by high root density in agricultural contexts likely acts as a major limit on crop yield. This project is thus highly timely, as understanding this phenomenon could unlock rapid increases in yield.Experimental ApproachTo assess the role of strigolactone in root density perception, we will use existing molecular genetic tools in the model plant Arabidopsis and in the crop species pea and barley, including strigolactone mutants and reporter lines. To identify new exudates that regulate plant-plant communication, we will collect exudates from barley and fractionate them, and use mass spectrometry to identify possible candidate signalling molecules. To understand the mechanisms by which plants to respond these signals, we will screen a barley diversity panel to perform QTL mapping for genes that regulate the response to root density. We will then identify and characterise the function of these genes.

背景植物可以检测根际根的密度，并利用该信息来确定根和芽生长的时空分配。植物检测和响应根密度的机制尚不清楚，但生化根分泌物可能发挥重要作用。独脚金内酯是一种抑制根和芽系统生长的激素，但也会渗出到土壤中；因此，它们是根密度感知的极好的候选信号分子。评估独脚金内酯在根密度感知和响应中的作用。2．识别在根际植物通讯中起作用的新型分泌物。3．了解植物对高根密度的反应机制。新颖性虽然根际植物通讯是一种长期被认可的现象，但很少有工作来揭示其背后的分子信号传导机制。因此，该项目代表了对重要生物现象的新研究路线。及时性在农业环境中，高根密度对芽生长的抑制可能是作物产量的主要限制。因此，该项目非常及时，因为了解这种现象可以实现产量的快速增加。实验方法为了评估独脚金内酯在根密度感知中的作用，我们将在模型植物拟南芥以及作物物种豌豆和大麦中使用现有的分子遗传工具，包括独脚金内酯突变体和报告系。为了识别调节植物间通讯的新分泌物，我们将从大麦中收集分泌物并将其分级，并使用质谱法来识别可能的候选信号分子。为了了解植物响应这些信号的机制，我们将筛选大麦多样性面板，以对调节根密度响应的基因进行 QTL 定位。然后我们将识别并表征这些基因的功能。