CORE--TRANSGENIC MOUSE/EMBRYONIC STEM CELL

核心--转基因小鼠/胚胎干细胞

• 批准号: 6563713
• 负责人: Lutz Birnbaumer
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563713
• 关键词: biomedical facility; embryonic stem cell; genetically modified animals; laboratory mouse

Description: The UCLA Transgenic Mouse/Embryonic Stem (ES) Cell Shared Resource was established to accommodate individual investigator?s needs in the production of transgenic and knockout mice and to provide for rederivation of strains to create specific pathogen-free mice. Resource personnel serve as consultants to many different investigations providing information on specific transgenic animal models, the design and assembly of DNA constructs, and the development of new methods. Materials can also be provided to investigators who wish to perform projects in their own laboratories. There are two separate components of the resource: Transgenic Mouse and ES Cell Culture. The Transgenic Mouse Facility was established in 1991. Although not funded by NCI in the last competitive renewal application, the institution has subsidized the facility?s operation because access to transgenic and knockout technology plays a vital role in the Cancer Center?s research mission. The facility performs pronuclear injections of DNA and blastocyst injections of targeted ES cells, rederives mouse strains, and maintains transgenic animal lines in their initial stages. The Embryonic Stem Cell Facility is not yet operational. Institutional funds have been utilized to recruit a director, renovate space, and provide equipment for the facility. Once operational, a formal service for culture, electroporation, drug selection, and expansion of ES clones will be available. The measure of usage is based on injection days for the transgenic facility and targeting projects for the ES facility. The total capacity for the transgenic facility is 240 injection days and for the ES facility, 25 cell targeting projects. Twenty four UCLA faculty members used the resource representing assistance to 66 individual projects. Of these, use by peerreviewed Cancer Center members totaled 60 percent. The total operating budget of the shared resource for 1997-1998 is $259,672, with 25 percent requested, 50 percent to be obtained from AIDS chargebacks, and 25 percent from institutional support.

描述：UCLA转基因鼠标/胚胎词干（ES）单元 建立了资源以适应个人调查员的需求 产生转基因和敲除小鼠，并提供重新培养 菌株创建特定的无病原体小鼠。资源人员作为 许多不同调查的顾问提供有关特定信息的信息 转基因动物模型，DNA构建体的设计和组装，以及 开发新方法。也可以向调查人员提供材料 希望在自己的实验室中执行项目的人。有两个 资源的单独组成部分：转基因小鼠和ES细胞培养。 转基因小鼠设施建立于1991年。 NCI在上次竞争性更新申请中，该机构有 补贴了该设施的操作，因为获得了转基因和淘汰 技术在癌症中心的研究任务中起着至关重要的作用。这 设施对DNA和胚泡注射的前注射 靶向ES细胞，重新生长小鼠菌株并保持转基因动物 线条处于初始阶段。 胚胎干细胞设施尚未运行。机构资金 已被用来招募董事，翻新空间并提供 设施的设备。一旦运作，是文化的正式服务， 将提供ES克隆的电穿孔，药物选择和扩展。 使用的度量是基于转基因设施的注射天数 并针对ES设施的项目。总能力 转基因设施为240天，对于ES设施，25个电池 定位项目。二十四名UCLA教职员工使用了资源 代表66个个人项目的帮助。其中，使用PeerReview 癌症中心成员总数为60％。总运营预算 1997 - 1998年共享资源的价格为259,672美元，要求25％ 从艾滋病拒绝获得的50％，从 机构支持。