CRYSTALLOGRAPHIC STUDIES OF MYOSIN SUBFRAGMENT 1

肌球蛋白亚片段 1 的晶体学研究

• 批准号: 6658537
• 负责人: IVAN RAYMENT
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658537
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; musculoskeletal system; proteins; radiography; structural biology

The objective of this research is to improve our understanding of the molecular basis of muscle contraction by continuing our studies of the three-dimensional structure of myosin subfragment-1 using single-crystal x-ray diffraction. Myosin is the major protein in muscle, in which it plays both a structural and enzymatic role. The myosin rod forms the backbone of the thick filament, whereas the myosin head is responsible for the generation of movement through the hydrolysis of ATP and its interaction with actin. The long term goal is to characterize all of the conformational intermediates in the contractile cycle which should then form the basis of a molecular mechanism of myosin-based motility.

这项研究的目的是提高我们对 通过继续我们的研究来了解肌肉收缩的分子基础 肌球蛋白亚片段 1 的三维结构 单晶X射线衍射。 肌球蛋白是体内的主要蛋白质 肌肉，它在其中发挥结构和酶的作用。 这 肌球蛋白杆形成粗丝的主干，而 肌球蛋白头负责通过 ATP 的水解及其与肌动蛋白的相互作用。 长期目标 是表征所有构象中间体 收缩周期应构成分子的基础 基于肌球蛋白的运动机制。