XRAY ABSORPTION SPECTROSCOPY OF METAL SUBSTITUTED IRON SULFUR CLUSTER

金属取代铁硫簇的X射线吸收光谱

• 批准号: 6586710
• 负责人: Michael W. Adams
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586710
• 关键词: biomaterials; biomedical resource; proteins; spectrometry; structural biology

Pyrococcus furiosus ferredoxin is unique among ferredoxins in having a single Fe4S4 cluster with one iron possessing non-sulfur coordination. An unusual feature of the protein is the ease with which the cluster can lose this iron to form a Fe3S4 ferredoxin. The Fe3S4 ferredoxin can readily be reconstituted with Fe2+ to reform the native Fe4S4 cluster, or alternatively with other metals ions to generate a mixed-metal iron sulfur cluster MFe3S4. Mixed metal iron sulfur clusters comprise a very important class of metalloprotein active sites and are of considerable interest. We propose to use XAS to systematically probe the electronic and physical structure of the different MFe3S4 clusters in Pyrococcus furiosus ferredoxin.

Ferredoxin的富氧蛋白在铁蛋白中是独一无二的 具有一个具有非硫的铁的单个Fe4s4簇 协调。 该蛋白质的不寻常特征是轻松 簇可以丢失这种铁以形成Fe3S4铁蛋白。 这 FE3S4铁氧还氧还可以与FE2+一起重组以改革 本机Fe4s4簇，或与其他金属离子或 产生混合金属铁硫簇MFE3S4。 混合金属铁 硫簇包含一类非常重要的金属蛋白 活跃的站点，引起了极大的兴趣。 我们建议使用XAS 系统地探究电子和物理结构 Ferredoxin中毒蛋白中的毒素中的不同MFE3S4簇。