XRAY DIFFRACTION OF MEMBRANE PROTEIN COMPLEX CRYSTALS MITOCHONDRIAL CYTOCHROME

膜蛋白复合物晶体线粒体细胞色素的 X 射线衍射

• 批准号: 6586566
• 负责人: CHANG-AN YU
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586566
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

The mitochondrial cytochrome b-c1 complex is an important electron transfer complex in the mitochondrial bioenergetic apparatus. The purified complex is composed of 10 protein subunits and about 20% of phospholipids. There are two cytochrome b one cytochrome c one iron-sulfur cluster and one ubiquinone in the complex. The molecular weight was calculated to be 236,000 dalton. The complex was crystallized in PEG in the presence of heptantriol and detergent. The crystals were stable at cold temperatures and they diffract x-rays to about 7 E resolution using a laboratory source and area detector. The crystals diffract to 4.5 E using a synchrotron source and rotation camera. Our newly improved crystals diffract to 2.9 E the using synchrotron source and rotation camera at SSRL. We wish to collect a complete data set on native crystals and start to do some screening on heavy atom derivatives and solve the structure in two years.

线粒体细胞色素b-c1复合物是一种重要的电子 线粒体生物能量装置中的转移复合物。 这 纯化的复合物由 10 个蛋白质亚基和约 20% 的蛋白质组成。 磷脂。 有两种细胞色素b 一种细胞色素c 一种 络合物中含有铁硫簇和一个泛醌。 分子 重量经计算为 236,000 道尔顿。 该建筑群是 在庚三醇和洗涤剂存在下在 PEG 中结晶。 这 晶体在低温下很稳定，它们会衍射 X 射线 使用实验室源和区域探测器时分辨率约为 7 E。 这 使用同步加速器源和旋转，晶体衍射至 4.5 E 相机。 我们新改进的晶体使用时可衍射至 2.9 E SSRL 的同步加速器源和旋转相机。 我们希望收集一个 完成天然晶体的数据集并开始进行一些筛选 重原子衍生并在两年内解决结构。