VERY HIGH RESOLUTION OF MANGANESE PEROXIDASE FROM PHANEROCHAETE CHRYSOSPORIUM

非常高分辨率地测定金孢原毛毛菌的锰过氧化酶

• 批准号: 6658709
• 负责人: MUNIRATHINAM SUNDARAMOORTHY
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658709
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; proteins; radiography; structural biology

Ribosomes are the complex assemblages of proteins and RNA chains which are responsible for the synthesis of proteins. Low-resolution structural maps, which have been generated by merging the data from many separate structural studies, have not been sufficient to elucidate many of the ribosome's most important structure/function relations. Only atomic-resolution structure of the ribosome can answer these questions. We plan to grow crystals of ribosomal subunits from cultured human (HeLa) cells. Subunits have been purified, and crystallization conditions will soon be tested.

核糖体是蛋白质和RNA链的复杂组合 负责蛋白质的合成。 低分辨率 结构图，通过合并数据来生成 许多单独的结构研究不足以 阐明许多核糖体最重要的结构/功能 关系。 仅核糖体的原子分辨率结构 回答这些问题。 我们计划种植核糖体的晶体 来自培养的人（HeLa）细胞的亚基。 亚基一直是 纯化，结晶条件将很快进行测试。