XRAY CRYSTALLOGRAPHIC STUDY OF THETA CLASS GLUTATHIONE S TRANSFERASE

Theta 类谷胱甘肽 S 转移酶的 X 射线晶体学研究

• 批准号: 6658610
• 负责人: CHRISTOPHER M BRUNS
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658610
• 关键词: Mammalia; bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Glutathione S-transferases (GSTs) are detoxification enzymes which modify and solubilize electrophilic toxins. These enzymes remove carcinogenic substances from the bloodstream and are involved in the breakdown of some therapeutic drugs. Thus they can play a role in cancer prevention and drug delivery. So far, researchers have failed to crystallize a mammalian theta class GST. We have succeeded in crystallizing the theta class GST 12-12 from rat liver. Due to the extreme radiation sensitivity of these unique crystals, a combination of synchrotron radiation and crystal cooling are likely to greatly improve the data that can be generated from them.

谷胱甘肽 S-转移酶 (GST) 是解毒酶， 修饰和溶解亲电子毒素。 这些酶可以去除 血液中的致癌物质并参与 一些治疗药物的分解。 因此他们可以发挥作用 癌症预防和药物输送。 迄今为止，研究人员尚未成功 结晶哺乳动物 theta 类 GST。 我们已经成功地 从大鼠肝脏中结晶出 theta 类 GST 12-12。 由于 这些独特晶体的极端辐射敏感性，组合 同步加速器辐射和晶体冷却可能会极大地 改进可以从中生成的数据。