Core--Morphology and biomechanics

核心--形态学与生物力学

• 批准号: 6592113
• 负责人: Noreen J Hickok
• 金额: $ 15.53万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-10 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592113
• 关键词: biomechanics; biomedical facility; confocal scanning microscopy; extracellular matrix; histology; immunocytochemistry; in situ hybridization; morphometry

DESCRIPTION (provided by applicant): Osteoarthritis is characterized by the progressive degeneration of the articular cartilage of the joints, ultimately leading to inflammation, pain, and impairment of mobility. In this proposal, we will address the molecular biology of osteoarthritis by means of manipulation of an in vitro chondrogenic cell culture system, application of gene knock-in technology to generate and then characterize mice that express mutated forms of cartilage types II and IX, and cartilage oligomeric matrix protein (COMP), and identification of candidate genes involved in cartilage development and maturation by means of differential gene expression profiling. The objective of the Morphology/Biomechanics Core (Core Leader: Rocky S. Tuan) is to provide state-of-the-art facilities and technologies for the characterization of the morphological, structural, gene expression profiling, and mechanical property testing of the cells and tissues generated from the Research Projects of the Program Project. The Morphology Component of the Core, will be responsible for routine histology, immunohistochemistry, in situ hybridization, ultrastructure, and confocal laser scanning microscopy, and will be carried out at the Orthopaedic Research Laboratory at Thomas Jefferson University. The Biomechanics Component will be supervised by Dr. Lori Setton in the Mechanical Engineering Department at Duke University, and will be responsible for the application of the osmotic loading technique to determine the mechanical properties of joint articular cartilage in the experimental mouse models that harbor the extracellular matrix gene mutations. Both Components of the core are staffed by experienced investigators with established, published experience in the application of these techniques in cartilage cells and tissues. The Morphology/Biomechanics Core therefore serves a vital function in the end-point analysis of cartilage structure and function, and is essential for the successful completion of the studies proposed in each of the Research Projects.

描述（由申请人提供）： 骨关节炎的特征是 关节的关节软骨，最终导致炎症，疼痛， 和流动性障碍。在此提案中，我们将解决分子 骨关节炎的生物学通过操纵体外软骨的生物学 细胞培养系统，基因敲门技术的应用和 然后表征表达软骨II型和IX的突变形式的小鼠， 和软骨寡聚基质蛋白（COMP）以及候选者的鉴定 通过差异涉及软骨发育和成熟的基因 基因表达分析。形态/生物力学核心的目的 （核心负责人：Rocky S. Tuan）将提供最先进的设施 形态，结构，基因表征的技术 表达分析和细胞和组织的机械性能测试 由计划项目的研究项目产生。形态 核心的组成部分将负责日常工作 组织学，免疫组织化学，原位杂交，超微结构和 共聚焦激光扫描显微镜，将在骨科进行 托马斯·杰斐逊大学的研究实验室。生物力学组成部分 将由机械工程部门的洛里·塞顿（Lori Setton）博士监督 在杜克大学，将负责应用渗透 加载技术以确定关节关节的机械性能 在具有细胞外基质的实验小鼠模型中的软骨 基因突变。核心的两个组成部分均由经验丰富 具有既定，已发表经验的调查员 这些技术在软骨细胞和组织中。形态/生物力学 因此，核心在软骨的终点分析中起着至关重要的功能 结构和功能，对于成功完成 在每个研究项目中提出的研究。