COMPLEX MIXTURE DISCRIMINATION IN OLFACTORY SIGNALING

嗅觉信号中的复杂混合物辨别

• 批准号: 6628402
• 负责人: CHARLES E LINN
• 金额: $ 24.7万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6628402
• 关键词: Lepidoptera; afferent nerve; axon; behavior test; central neural pathway /tract; chemoreceptors; gender difference; histology; imaginal disc; invertebrate endocrinology; morphometry; neural information processing; neuroanatomy; neurogenesis; neurons; neurophysiology; neuroregulation; olfactions; olfactory stimulus; pheromone; sensory discrimination; sensory signal detection; single cell analysis; xenotransplantation

DESCRIPTION: (Adapted from the Investigator's Abstract) Olfactory glomeruli are the common functional elements in vertebrate and invertebrate neural pathways involved in integrating sensory information about complex odor mixtures. Previous studies have shown that insects can be excellent models for studying the functional role of glomeruli. One of the most studied involves the sex pheromone-processing pathways in the male-specific macroglomerular complex (MGC) of the antennal lobe of male moths. Olfactory glomeruli in the MGC are critically involved in the spatial organization of sensory axons for specific pheromone components and their interactions with specific local interneurons and projection neurons that integrate key aspects of odor quality, quantity, and intermittency. The objectives of the present proposal are to test hypotheses about the interactions of sensory afferents and central neuron types in the olfactory glomeruli, utilizing the technique of transplantation of antennal imaginal disks between males and females of two heliothine moths, the tobacco budworm, Heliothis virescens, and the corn earworm, Helicoverpa zea. These two insect species have been the subject of extensive studies on pheromone blend discrimination, and electrophysiological/anatomical studies of neural pathways involved in processing the pheromone signal. The antennal transplant procedure will allow construction of males and females with unique antennal and behavioral response phenotypes to test a model for glomerular organization and behavioral discrimination of odor quality. These inter-species antennal transplants represent a new experimental paradigm for studying basic aspects of the structural and functional role of glomeruli in olfactory discrimination.

描述：（改编自研究者的摘要） 嗅觉肾小球是脊椎动物中常见的功能元件 无脊椎动物的神经通路参与整合感觉信息 复杂的气味混合物。此前的研究表明，昆虫可以 研究肾小球功能作用的优秀模型。最有之一 研究涉及男性特异性的性信息素处理途径 雄蛾触角叶的大肾小球复合体（MGC）。嗅觉 MGC 中的肾小球在空间组织中发挥着重要作用 特定信息素成分的感觉轴突及其与 整合关键方面的特定局部中间神经元和投射神经元 气味的质量、数量和间歇性。当前的目标 建议测试关于感觉传入和感觉传入相互作用的假设 嗅觉肾小球中枢神经元类型，利用技术 两只雄性和雌性触角成虫盘移植 夜蛾、烟草芽虫、烟芽夜蛾和玉米 耳虫，Helicoverpa zea。这两种昆虫已成为研究对象 对信息素混合辨别的广泛研究，以及 涉及神经通路的电生理学/解剖学研究 处理信息素信号。触角移植手术将允许 构建具有独特触角和行为反应的雄性和雌性 表型来测试肾小球组织和行为模型 气味质量的辨别。这些跨物种触角移植 代表了研究基本方面的新实验范式 肾小球在嗅觉辨别中的结构和功能作用。