GLYCOSYL COMPOSITION ANALYSIS OF PORCINE INTESTINAL HEPARINS

猪肠肝素的糖基成分分析

• 批准号: 6653583
• 负责人: STEVEN B LEVERY
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653583
• 关键词: biological products; biomedical resource; carbohydrates; lipids; nuclear magnetic resonance spectroscopy; spectrometry

1H-NMR analysis was performed on a Varian Inova 600-MHz spectrometer. The sample was deuterium-exchanged by lyophilization from D2O and dissolved in 0.5 mL DMSO-d6/D2O 982. The 1D 1H-NMR spectrum was obtained at 308(K (35(C) at 600 MHz using presaturation of the residual HOD signal. Chemical shifts were measured relative to the residual protonated DMSO-d6 at 2.503 ppm (measured indirectly relative to TMS=0.0ppm). The positive ion mode FAB mass spectrum was obtained using the first two sectors of a JEOL SX/SX102A tandem four-sector mass spectrometer operated at an acceleration potential of 10 kV. Linear upward scanning was employed such that the mass range from m/z 100 to 2500 is scanned in 1 minute. Application of both techniques confirmed the identity of the ganglioside with respect to the structure of the glycan, the molecular weight distribution and type of ceramides present, and the overall purity.

1H-NMR分析在Varian Inova 600-MHz上进行 光谱仪。 样品是通过冻干的氘化处理的 从D2O并溶解在0.5 mL DMSO-D6/D2O 982中。1D1H-NMR 在308（k（35（c））在600 MHz时，使用预饱和度获得了频谱 残留的HOD信号。 相对于 在2.503 ppm处的残留质子化DMSO-D6（间接测量 相对于TMS = 0.0ppm）。 正离子模式Fab质谱是 使用Jeol SX/SX102A串联的前两个扇区获得 四个扇区质谱仪以加速电位运行 10 kV。 使用线性向上扫描，使质量范围 从m/z 100到2500在1分钟内扫描。 两者的应用 技术证实了神经苷的身份 聚糖的结构，分子量分布和 神经酰胺的类型和整体纯度。