CORE--MOUSE FACILITY

核心——鼠标设施

• 批准号: 6564222
• 负责人: EDWARD M RUBIN
• 金额: $ 14.33万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564222
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse; protein 4.1; tissue /cell culture

The mouse core will support the various components of the program in two major ways: a) the preparation of transgenic and gene knockout mice; and, b) the maintenance of various mutant mouse lines to be used in the proposed studies. A major initial focus of the core will relate to the creation of both protein 4.1 knockout mice and mice expressing various protein 4.1 transgenes. The proposed studies to dissect in vivo the biological functions of protein 4.1 are described in projects 1, 2, 3, and 6. The core will play a central role in assisting the participating programs in designing the various aspects involved in of the murine studies. All the technical steps in creating the transgenic and gene knock- out mice (i.e. creating the various DNA constructs and performing embryo manipulations) will be carried out as part of this core. The core will also maintain and provide thalassemic mice.

鼠标核心将在两个中支持程序的各个组件 主要方法：a）转基因和基因敲除小鼠的制备； ，b）维护各种突变小鼠线 拟议的研究。 核心的主要初始重点将与 创建蛋白质4.1敲除小鼠和表达各种蛋白质的小鼠 蛋白4.1转基因。 拟议的研究是在体内剖析的 蛋白质4.1的生物学功能在项目1、2、3中描述 和6。核心将在协助参与的核心角色中发挥核心作用 设计鼠涉及的各个方面的程序 研究。 创建转基因和基因的所有技术步骤 敲除小鼠（即创建各种DNA构建体并执行 胚胎操作将作为该核心的一部分进行。 这 核心还将维持并提供丘脑小鼠。