BIACORE 3000 Biosensor

BIACORE 3000 生物传感器

• 批准号: 6440256
• 负责人: Stanley Richard Terlecky
• 金额: $ 27万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440256
• 关键词: biomedical equipment purchase; chemical kinetics; intermolecular interaction; surface plasmon resonance

DESCRIPTION (provided by the applicant): The goal of this proposal is to bring a BIAcore 3000 biosensor to the Wayne State University. This instrument, designed to detect, monitor, and quantify biomolecular interactions, will be a significant asset to the research programs of a number of NIH-funded investigators at this institution. At present, there is no biosensor available at Wayne State nor anywhere else in metropolitan Detroit. The instrument will be of great benefit to the applicants in a variety of ongoing projects including i. an examination of the interactions among proteins involved in peroxisome biogenesis, ii. a quantification of estrogen receptor (and mutated estrogen receptor)-.coregulator interactions, iii. a characterization of the interactions of cathepsins and extra-cellular matrix proteins in the context of proteolytic cascades which occur on the tumor cell surface, iv. a molecular description of how the estrogen receptor binds specific protein, DNA, and attenuated estrogen ligands, v. an examination of how the aryl hydrocarbon receptor binds the retinoblastoma tumor suppressor protein and mediates cell cycle control, and vi. an analysis of the interactions of matrix metalloproteinases and their inhibitors, called TIMPS, in cancer. Wayne State University is strongly committed to this proposal. Through the contributions of 19 investigators, the School of Medicine, and the University at large, the following have been secured: 1. A funded long-term plan for maintenance and continued operation of the BIAcore 3000 biosensor. 2. A centrally located core facility to house the equipment. 3. A funded technician who will be trained to operate and schedule usage of the instrument. The BIAcore 3000 biosensor is a state-of-the-art instrument that will provide extremely reliable data for years to come. The plan outlined herein will maximize efficient use of this instrument.

描述（由申请人提供）：该提案的目的是带来 韦恩州立大学的Biacore 3000生物传感器。这个乐器， 旨在检测，监测和量化生物分子相互作用，将是一个 许多NIH资助的研究计划的重要资产 该机构的调查人员。目前，没有可用的生物传感器 在韦恩州，底特律大都会的任何地方。 该仪器对申请人有很大的好处 正在进行的项目，包括i。检查蛋白质之间的相互作用 参与过氧化物酶体生物发生，ii。雌激素受体的定量 （和突变的雌激素受体） - 核心节相互作用，iii。一个 策展人与细胞外基质的相互作用的表征 在肿瘤细胞上发生的蛋白水解级联反应的蛋白质 表面，iv。雌激素受体如何结合的分子描述 特异性蛋白质，DNA和减弱的雌激素配体，v。 芳基烃受体如何结合视网膜母细胞瘤抑制剂 蛋白质并介导细胞周期控制和VI。分析 基质金属蛋白酶及其抑制剂的相互作用，称为TIMP， 在癌症中。 韦恩州立大学强烈致力于这一建议。通过 19位调查人员，医学院和大学的贡献 总的来说，以下是确保的： 1。一项资助的长期维护和继续运营的长期计划 Biacore 3000生物传感器。 2。位于中央的核心设施，用于容纳设备。 3。一名资助的技术人员，将接受培训以操作和安排使用 乐器。 Biacore 3000生物传感器是一种最先进的仪器 未来几年的数据非常可靠。本文概述的计划将 最大程度地利用该仪器。