MOLECULAR EXTENT OF LINKAGE DISEQUILIBRIUM

连锁不平衡的分子范围

• 批准号: 6582373
• 负责人: Kenneth K. KIDD
• 金额: $ 15.83万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6582373
• 关键词: Pongidae; animal genetic material tag; animal population genetics; cell line; centromere; denaturing gradient gel electrophoresis; gene frequency; genetic mapping; genetic markers; genetic polymorphism; genetic recombination; human genetic material tag; human population genetics; linkage disequilibriums; molecular genetics; nucleic acid repetitive sequence; nucleic acid sequence; restriction fragment length polymorphism; restriction mapping; single strand conformation polymorphism; telomere

DESCRIPTION: (Adapted from applicant?s abstract): This project proposes to systematically investigate linkage disequilibrium in human populations. Data to be collected consist of haplotypes spanning 200-500 kb at eight independent loci in 20 populations. The eight loci, which are a subset of the 20 loci investigated in Project 1, will include centromeric, telomeric, and mid-arm chromosomal regions, in order to investigate systematic influences of chromosomal regions on linkage disequilibrium. The 20 populations, which are identical to the 20 populations analyzed in Project 1, will comprise a representative sample of global human diversity, in order to minimize idiosyncratic influences of particular population histories on linkage disequilibrium. Initially, a number of polymorphic markers will be identified and typed in a 200 kb region of each locus. Additional markers will then be added to allow the total region to be spanned in each locus to be adjusted either upwards (to a maximum of 500 kb) or downwards, depending on the amount of linkage disequilibrium found in the initial survey of 200 kb from each locus. The stated goal is to evaluate the amount and distribution of recombination across each locus, and the relative influence of shared vs. independent population histories, in shaping the overall pattern of linkage disequilibrium across the 160 population-locus combinations.

描述：（改编自申请人的摘要）：该项目提议 系统地研究人口中的连锁不平衡。数据 要收集的单倍型在八个独立处跨越200-500 kb 20个人群中的基因座。八个基因座，是20个基因座的子集 在项目1中进行的调查将包括Centromeric，端粒和中臂 染色体区域，以研究 连锁不平衡的染色体区域。 20个人口 与项目1中分析的20个人群相同，将包括 全球人类多样性的代表性样本，以最大程度地减少 特定人口历史对联系的特质影响 不平衡。最初，将确定许多多态标记 并输入每个基因座的200 kb区域。然后，其他标记将是 添加以允许调整每个位点中的总区域 根据数量 从每个的最初调查中发现的200 kb的连锁不平衡 轨迹。既定的目标是评估 每个基因座的重组以及共享VS的相对影响。 独立的人口历史，塑造整体联系方式 在160个种群 - 局部组合中的不平衡。