Effect of red tide toxins on biomarker verification & epidemiology

赤潮毒素对生物标志物验证的影响

• 批准号: 6614506
• 负责人: LORA Elderkin FLEMING
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6614506
• 关键词: adolescence (12-20); aerosols; animal tissue; asthma; biomarker; chronic obstructive pulmonary disease; clinical research; disease /disorder proneness /risk; environmental exposure; environmental health; environmental toxicology; human old age (65+); human subject; immunocytochemistry; marine toxins; respiratory disorder; respiratory disorder epidemiology; respiratory function

(Description: (Taken from the Investigator's Abstract) Very little is published in the scientific literature on the human health effects of Florida, red tide, either as human clinical case receptors or formal epidemiological studies. In addition to the health effects associated with the ingestion of contaminated shellfish (i.e. neurotoxic shellfish poisoning NSP)), there have been multiple anecdotal reports of respiratory irritation and possibly immunologic effects associated with the inhalation of aerosolized Florida red tide toxins in recreational and occupational persons on the beaches on the Gulf of Mexico. Furthermore, recent die-offs of the endangered Florida manatee were associated with the inhalation of the Florida red tide toxins, and research in sheep and other laboratory animals has confirmed the ability of aerosolized red tide toxins to cause reversible bronchospasm. This proposed study will establish a human exposure and effect biomarker of aerosolized red tide toxins exposure in animals, and then pilot the use of the biomarker in several sensitive exposed human populations longitudinally. This biomarker of both exposure and effect consists of throat and nose swabs for inflammatory and mucosal cells before and after exposure to aerosolized Florida red tide toxins. The effect marker is the actual number of inflammatory and mucosal cells before and after exposure to aerosolized Florida red tide toxins. The effect marker is the actual number of inflammatory and mucosal cells, while the actual marker is the qualitative and quantitative measure of brevetoxin (the primary red tide toxin) in the inflammatory and mucosal cells using immunocytochemical staining. The investigators will work with the Immunocytochemistry Core and the animal model investigators to further elaborate the biomarker prior to extensive human testing. At the same time, the investigators will assemble several population cohorts for evaluation of the biomarkers in at risk exposure and possibly sensitive subpopulations. These populations include 2 sensitive persons with chronic lung disease. At the same time, in cooperation with the Centers for Diseases Control and Prevention (CDC), an occupationally exposed group consisting of lifeguards and similarly exposed workers will be studied. Survey instruments and measures of physiologic lung function will be evaluated, as well as personnel monitoring for the aerosolized red tide toxins gathered by the exposure investigators and the Toxin Core. The biomarker will be collected and analyzed in conjunction with the epidemiologic and clinical data, correlated with environmental/personnel exposure data, to better define the acute and chronic human health effects of aerosolized red tide toxins exposure in Florida. Ultimately, this research will contribute to the model of the animal and human health effects of aerosolized natural toxins elaborated in the Program Project proposal.

（描述：（摘自研究者摘要）关于佛罗里达赤潮对人类健康影响的科学文献很少，无论是作为人类临床病例受体还是正式的流行病学研究。除了与摄入相关的健康影响之外受污染的贝类（即神经毒性贝类中毒 NSP）），已有多起关于呼吸道刺激的轶事报道，并可能与在娱乐场所吸入雾化的佛罗里达赤潮毒素有关。以及墨西哥湾海滩上的职业人员。此外，最近濒临灭绝的佛罗里达海牛的死亡与吸入佛罗里达赤潮毒素有关，对绵羊和其他实验动物的研究已经证实，雾化的赤潮毒素能够引起可逆性支气管痉挛。这项拟议的研究将建立人类暴露和动物气溶胶赤潮毒素暴露影响的生物标志物，然后在几个敏感暴露人群中纵向试点使用该生物标志物。这种暴露和影响的生物标志物由暴露于雾化佛罗里达赤潮毒素之前和之后的咽喉和鼻拭子的炎症和粘膜细胞组成。效应标记是接触雾化佛罗里达赤潮毒素之前和之后炎症和粘膜细胞的实际数量。效应标记物是炎症细胞和粘膜细胞的实际数量，而实际标记物是利用免疫细胞化学染色对炎症细胞和粘膜细胞中的短尾藻毒素（初级赤潮毒素）进行定性和定量测量。研究人员将与免疫细胞化学核心和动物模型研究人员合作，在广泛的人体测试之前进一步阐述生物标志物。与此同时，研究人员将聚集几个人群队列，以评估高危暴露人群和可能敏感亚人群的生物标志物。这些人群包括 2 名患有慢性肺病的敏感人群。同时，将与美国疾病控制与预防中心（CDC）合作，对由救生员和类似暴露工人组成的职业暴露群体进行研究。将评估生理肺功能的调查仪器和测量方法，以及对暴露调查人员和毒素核心收集的气溶胶赤潮毒素的人员监测。将结合流行病学和临床数据以及环境/人员暴露数据来收集和分析生物标志物，以更好地确定佛罗里达州气溶胶赤潮毒素暴露对人类健康的急性和慢性影响。最终，这项研究将有助于建立计划项目提案中阐述的雾化天然毒素对动物和人类健康影响的模型。