HISTOLOGICAL VISUALIZATION & ASSESSMENT: CONFOCAL MICROSCOPY

组织学可视化

• 批准号: 6660150
• 负责人: RICHARD A ALTSCHULER
• 金额: $ 20.08万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6660150
• 关键词: Mammalia; animal tissue; bioengineering /biomedical engineering; biomaterials; biomedical resource; ear; hearing; medical rehabilitation related tag; nervous system; prosthesis; technology /technique

A critical component of implantable micro-probe development and in vivo testing is the assessment of the probe in its tissue environment, which we term the morphological substrate of function. This helps to determine if the probe is causing any damage to the surrounding tissue. Just as importantly, it allows one to identify and characterize the cellular elements in the tissue that are interacting with the probe. The objectives in this project are to increase our ability to assess the interaction of the probe with its tissue substrate both in the recording mode and for stimulation. Our first objective is to further characterize the cells the probe is recording from by combining physical information with geometric information in hopes of identifying the actual cells involved in chronic recordings. For stimulation experiments, we hope to better characterize tissue reaction to the probe and to the charge flow introduced through chronic electrical stimulation. Our second objective is to generate quantitative information on cellular elements. This will allow us to characterize and compare tissue reaction with different probes and different conditions as well as to better define the normal environment the probe is operating in. Finally, our third objective is to assess and visualize in three dimensions which will support objectives on other project which involve modeling of complex patterns of tissue reaction surrounding the probe structures. All of these objectives will provide valuable feedback towards improving electrode designs.

植入式微探针开发和应用的关键组成部分 体内测试是对探针在其组织环境中的评估， 我们称之为功能的形态基础。 这有助于 确定探头是否对周围环境造成任何损害 组织。 同样重要的是，它允许人们识别和 表征组织中相互作用的细胞元素 与探头。 该项目的目标是提高我们的 评估探针与其组织相互作用的能力 基板在记录模式和刺激。 我们的第一个 目的是进一步表征探针正在记录的细胞 通过将物理信息与几何信息相结合 希望能够识别出参与慢性记录的实际细胞。 对于刺激实验，我们希望更好地表征组织 对探针和通过引入的电荷流的反应 慢性电刺激。 我们的第二个目标是产生 细胞元素的定量信息。 这将使我们能够 表征和比较不同探针的组织​​反应 不同的条件以及更好地定义正常 探测器运行的环境。最后，我们的第三个目标 是在三个维度上进行评估和可视化，这将支持 其他涉及复杂模式建模的项目的目标 探针结构周围的组织反应。 所有这些 目标将为改进电极提供有价值的反馈 设计。