Population Genetics of Reproductive Protein Diversity

生殖蛋白质多样性的群体遗传学

• 批准号: 6617568
• 负责人: WILLIE J SWANSON
• 金额: $ 33.49万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6617568
• 关键词: Drosophilidae; Gastropoda; Mammalia; SDS polyacrylamide gel electrophoresis; affinity chromatography; biochemical evolution; differential display technique; egg proteins; female reproductive system; fertility; gene expression; genetic library; genetic polymorphism; male reproductive system; microarray technology; molecular shape; northern blottings; nucleic acid sequence; polymerase chain reaction; population genetics; protein protein interaction; sequence tagged sites; species difference; sperm; yeast two hybrid system

DESCRIPTION (provided by applicant): The long-term goal of this project is to understand the diversity of reproductive proteins and the functional consequences of their divergence, which could involve problems in fertility and speciation due to a mismatch of sperm-egg recognition molecules. A recurring observation in the study of reproductive proteins is their rapid, adaptive evolution. This phenomenon occurs in organisms diverse as diatoms and mammals. The research proposed here is aimed at identifying interacting male-female reproductive proteins and studying their co-evolution in three systems: Drosophila, abalone, and mammals. Specific Aim 1 is to identify cognate male-female pairs of reproductive proteins in Drosophila, where no interacting male-female reproductive proteins are known. This will greatly increase the use of Drosophila as a model organism for the study of reproductive proteins. Specific Aim 2 is to use abalone to test the functional consequences of diversity in reproductive proteins using a well-established interaction of sperm and egg molecules. This is not currently possible in other animal systems. Specific Aim 3 uses the knowledge gained from the study of reproductive protein diversity in other systems to test for correlated changes in sperm-egg recognition molecules from a pool of proposed interacting mammalian sperm-egg molecules. The identification of interacting mammalian sperm-egg molecules is a long-standing controversy in mammalian fertilization. Our approach, a direct application of results and ideas from basic research, is a novel comparative genomics analysis aimed at testing the proposed interactions.

描述（由申请人提供）：该项目的长期目标是了解生殖蛋白的多样性及其差异的功能后果，这可能会涉及由于精子 - 埃格识别分子不匹配而导致的生育和物种物种问题。生殖蛋白研究中的反复观察是它们的快速，适应性进化。这种现象发生在各种生物体中，如硅藻和哺乳动物。此处提出的研究旨在鉴定相互作用的男女生殖蛋白，并研究其在三个系统中的共同进化：果蝇，鲍鱼和哺乳动物。 具体目的1是鉴定果蝇中的同志女性女性生殖蛋白对，在果蝇中，尚无相互作用的男性生殖蛋白。这将大大增加果蝇作为模型生殖蛋白的模型生物的使用。 具体目标2是使用鲍鱼使用精子和卵子分子的相互作用来测试生殖蛋白质多样性的功能后果。目前在其他动物系统中是不可能的。具体目标3使用从其他系统中生殖蛋白多样性的研究中获得的知识来测试从提出的相互作用的哺乳动物精子 - 卵形分子库中的精子 - 蛋识别分子的相关变化。相互作用的哺乳动物精子分子相互作用是哺乳动物施肥的长期争议。我们的方法是基础研究的结果和想法的直接应用，是一种旨在测试拟议相互作用的新型比较基因组学分析。