CORE--NEUROPATHOLOGY AND TISSUE CULTURE

核心——神经病理学和组织培养

• 批准号: 6825079
• 负责人: SIDNEY CROUL
• 金额: $ 25.98万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6825079
• 关键词: Papovaviridae; athymic mouse; biomedical facility; cell line; electron microscopy; histopathology; human tissue; immunocytochemistry; laboratory mouse; medulloblastoma; tissue /cell culture; tissue resource /registry; virus related neoplasm /cancer

Core B: Neuropathology and Tissue Culture Core JC virus (JCV) is well known as the cause of the human demyelinating disease progressive multifocal leukoencephalopathy (PML). In animals and in humans it has been associated with the production of brain tumors, most likely through mechanisms involving T-antigen a product of the JCV early genes. In human medulloblastomas, a frequent malignant brain tumor of children, our laboratory has also demonstrated JCV DNA and T antigen protein using PCR and immunocytochemistry. We have also developed a murine model of medulloblastoma in mice transgenic for the JCV archetypal early genes (JCV-CY mice). Because of the parallels between the human tumors and murine model, this program project proposes to focus on medulloblastomas and the relationship to JCV pathways. Within the overall project, Core B will perform pathologic evaluation of animals, tumors, and primary cultures from tumors generated by Core Component A: Experimental Animal Core. Core will also derive cell lines from primary tumor tissue and supply the individual projects with the necessary tissue and cell lines. This Core will contribute to the program project by providing a systematic, consistent and reliable mechanism for the tissue based analysis of the experimental animals, tumors and cell cultures utilized in the experiments. Integrating the pathologic and cell culture expertise with Projects 1,2 and 3 plus Core A will ensure that handling, storage and analysis of these specimens and cells is uniform and efficient.

核心B：神经病理学和组织培养核心 JC病毒（JCV）众所周知，是人类脱髓鞘疾病进行性多灶性白血病（PML）的原因。在动物和人类中，它与脑肿瘤的产生有关，这很可能是通过涉及T-抗原的机制作为JCV早期基因的产物。在人类髓母细胞瘤是儿童的常见恶性脑肿瘤中，我们的实验室还使用PCR和免疫细胞化学证明了JCV DNA和T抗原蛋白。我们还开发了一种针对JCV原型早期基因（JCV-CY小鼠）的小鼠转基因的髓母细胞瘤模型。由于相似之处 在人类肿瘤和鼠模型之间，该计划项目提议关注髓母细胞瘤以及与JCV途径的关系。在整个项目中，核心B将对由核心成分A：实验性动物核心产生的肿瘤的动物，肿瘤和原发性培养物进行病理评估。核心还将从原发性肿瘤组织中得出细胞系，并为各个项目提供必要的组织和细胞系。该核心将通过为实验中使用的实验动物，肿瘤和细胞培养物提供系统的系统，一致和可靠的机制来为该计划项目做出贡献。将病理和细胞培养专业知识与项目1,2和3集成 再加上核心A将确保对这些标本和细胞的处理，存储和分析均匀有效。