Core--Neuropathology and Cell Culture

核心--神经病理学和细胞培养

• 批准号: 6259210
• 负责人: SIDNEY CROUL
• 金额: $ 28.78万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6259210
• 关键词: AIDS /HIV neuropathy; Polyomavirus hominis 2; animal tissue; astrocytes; biomedical facility; genetically modified animals; glia; human immunodeficiency virus 1; human tissue; laboratory mouse; neuropathology; oligodendroglia; tissue /cell culture; AIDS /HIV neuropathy; Polyomavirus hominis 2; animal tissue; astrocytes; biomedical facility; genetically modified animals; glia; human immunodeficiency virus 1; human tissue; laboratory mouse; neuropathology; oligodendroglia; tissue /cell culture

This is a proposal to establish a Neuropathology and Tissue Culture Core which will help the overall program to determine the pathological effects of HIV-1 and JCV in the brain and to elucidate the molecular pathways through which theses viruses interact. We will provide surgical and autopsy brain tissue samples from AIDS and non-AIDS PML patients diagnosed with HIV-1 encephalopathy, to be used primarily in Projects 1, 2 and 3, for the co-localization of HIV-1 or JCV proteins with cellular proteins relevant to viral activities. We will perform, in conjunction with Project 1, pathological analyses of tissue samples from mice transgenically expressing JCV T-antigen under the control of the JCV early promoter. We will provide highly purified cultures of microglial, oligodendrocytes and astrocytes from human fetal CNS tissue, and from human adult CNS tissue. As a service to all three projects, we will maintain cultures of established permanent cell lines, including the progenitor CG-4 cells, which will be utilized in Projects 1 and 2. Finally, we will provide quality control of primary cultures and cell lines, and evaluate the differentiation state of CG-4 cells by immunocytochemical staining with cell type- specific markers. The proposed services will help provide essential material for the three participant projects, aimed at deciphering the role of host regulatory proteins in interacting with HIV-1 and JCV viral factors in the pathogenesis off two important CNS disorders.

这是建立神经病理学和组织培养核心的一项建议，该提议将帮助整个程序确定HIV-1和JCV在大脑中的病理影响，并阐明这些病毒相互作用的分子途径。我们将提供来自诊断为HIV-1脑病的艾滋病和非AIDS PML患者的外科和尸检脑组织样品，主要用于项目1、2和3，用于与病毒活性相关的细胞蛋白的HIV-1或JCV蛋白共定位。我们将与项目1结合进行，对JCV早期启动子的控制，对从转基因表达JCV T-抗原的小鼠的组织样品进行病理分析。我们将提供来自人类胎儿中枢神经系统组织以及人类成人中枢神经系统组织的小胶质细胞，少突胶质细胞和星形胶质细胞的高度纯化培养物。作为对所有三个项目的服务，我们将维护已建立的永久细胞系的培养物，包括祖细胞CG-4细胞，该细胞将在项目1和2中使用。最后，我们将通过与细胞类型特定标记物进行Immunocycytosical syparsing cg-4细胞的质量控制，并评估CG-4细胞的分化状态。拟议的服务将有助于为这三个参与者项目提供必要的材料，旨在破译宿主调节蛋白在与HIV-1和JCV病毒因素相互作用中的作用中，从两种重要的CNS疾病中的发病机理中。