C11 ACETATE PET IMAGING OF PROSTATE AND RENAL CANCER

前列腺癌和肾癌的 C11 醋酸盐 PET 成像

• 批准号: 6633914
• 负责人: SCOTT E SNYDER
• 金额: $ 31.33万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6633914
• 关键词: HMG coA reductases; acetates; acetyl coA carboxylase; adenocarcinoma; athymic mouse; carbon; cell line; clinical research; diagnosis design /evaluation; fatty acid synthase; human subject; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; pancreas neoplasms; pharmacokinetics; positron emission tomography; prostate neoplasms; radionuclides; renal cell carcinoma; small cell lung cancer

DESCRIPTION: (Adapted from PI's Summary) There is a need for new approaches to metabolic imaging of prostate and renal cancer. Positron emission tomography (PET) allows for cross sectional metabolic imaging, and is now emerging as an important clinical diagnostic imaging modality. PET imaging of glycolytic metabolism using the tracer fluorodeoxyglucose has proven useful in the diagnosis and staging of several malignancies, but has shown limited value in renal and prostate cancer. Positron radiotracers depicting metabolic pathways other than glycolytic metabolism are needed to fully develop the potential of PET in oncology. Carbon-11 acetate is a potential radiotracer for the depiction of the altered intermediary metabolism in malignant neoplasms. The purpose of this research is to understand the participating metabolic pathways responsible for the high uptake and retention of acetate radiotracer observed on PET imaging in certain malignant neoplasms, most notably renal cell carcinoma and prostate cancer. Acetate is a central molecule of intermediary metabolism with access to both catabolic and anabolic pathways. In this proposed research, the participating metabolic pathways and intermediate pools of acetate radiotracer will be investigated in vitro using cell culture and animal models of selected malignant neoplasms and tissue culture of pools will be correlated with measurements of the expression of enzymes involved in intermediary and anabolic lipid metabolism of acetate to understand the rate limiting steps and dominant tracer pools responsible for the uptake and retention of radiotracer acetate in certain malignancies. Kinetics of carbon-11 acetate on PET imaging of human subjects with prostate cancer and renal cancer prior to resection, and on micro PET imaging of athymic mice bearing xenographs of selected malignancies will be analyzed and compared to enzyme measurement of these tissues in vitro to determine the role of transport and intermediary metabolism enzyme expression in the tracer kinetics of renal and prostate cancer. The carbon-11 acetate imaging of human subjects with prostate cancer and renal cell carcinoma involved in this study will also be compared with surgical and histopathological findings to assess diagnostic accuracy in clinical diagnosis.

描述：（改编自PI的摘要）需要新方法 前列腺和肾癌的代谢成像。正电子发射断层扫描 （PET）允许进行横截面代谢成像，现在正在作为一个 重要的临床诊断成像方式。糖酵解的宠物成像 使用示踪剂氟脱氧葡萄糖的代谢已被证明在 几种恶性肿瘤的诊断和分期，但在 肾脏和前列腺癌。描绘代谢途径的正电子放射性示例 除了糖酵解代谢之外，还需要充分发展 宠物肿瘤学。碳11乙酸盐是描绘的潜在放射性示例 恶性肿瘤中中介代谢改变。目的 这项研究是了解负责的参与代谢途径 为了在PET上观察到的乙酸盐放射性示踪剂的高摄取和保留率 在某些恶性肿瘤中进行成像，最著名的是肾细胞癌和 前列腺癌。醋酸盐是中间代谢的中央分子 访问分解代谢和合成代谢途径。在这项拟议的研究中， 参与的代谢途径和乙酸盐放射性示踪剂的中间池 将在体外使用细胞培养和选定的动物模型在体外进行研究 池的恶性肿瘤和组织培养将与 涉及中间和合成代谢的酶表达的测量 乙酸的脂质代谢，以了解限制速率的步骤和占主导地位 示踪剂池负责吸收和保留乙酸盐剂 某些恶性肿瘤。人类PET成像的碳11乙酸酯动力学 切除前前列腺癌和肾癌的受试者以及微观 带有选定恶性肿瘤的无胸腺小鼠的宠物成像将是 分析并与这些组织在体外对这些组织的测量进行了比较 确定转运和中间代谢酶表达的作用 在肾脏和前列腺癌的示踪剂动力学中。乙酸碳11 患有前列腺癌和肾细胞癌的人类受试者的成像 参与这项研究也将与外科手术和 组织病理学发现，以评估临床诊断中的诊断准确性。