Epilepsy and Electrical Stimulation of the STN

癫痫和 STN 电刺激

• 批准号: 6646454
• 负责人: Fred Alexander Lado
• 金额: $ 13.15万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6646454
• 关键词: autoradiography; brain electrical activity; brain electronic stimulator; brain mapping; convulsions; electrophysiology; epilepsy; laboratory rat; nervous system disorder therapy; neurophysiology; neuroprotectants; nonhuman therapy evaluation; single cell analysis; subthalamus

DESCRIPTION (provided by applicant): This application is for a Mentored Clinical Scientist Development Award (K08) for Fred Lado, MD PhD. Dr. Lado obtained clinical training in neurology at the Cornell University Medical College and subsequently completed a fellowship in Clinical Neurophysiologywith emphasis in EEG and Epilepsy at the Albert Einstein College of Medicine. His current faculty appointment in the Department of Neurology at the Albert Einstein College of Medicine began in 1999. He previously received a PhD for his investigation of human subjects using magnetoencepahalography, but he is currently learning in vivo experimental methods using animals, as these are best suited to his longterm career goals. His goals are to study subcortical structures that regulate and propagate seizure activity, and to develop therapies targeting these regions in order to control human epilepsy. The Albert Einstein College of Medicine and the Montefiore Medical Center, the main teaching hospital of the College, offer broad strengths in basic neuroscience and clinical epileptology. Laboratory space, equipment, office space and access to established scientists across multiple disciplines are readily available. Opportunities for didactic instruction are also available through the medical college and through specialized summer courses. The environment and support available to the candidate are ideally suited to promoting and fostering Dr. Lado's longterm professional goals. Dr. Lado proposes to investigate the anticonvulsant effects of electrical stimulation of the subthalamic nucleus. The goals of these investigations are fourfold. (1) To investigate a novel treatment of seizures of adults using deep brain electrical stimulation of the subthalamic nucleus (STN). (2) To investigate the mechanisms of anticonvulsant action of STN stimulation and further development of our basic understanding of intrinsic anticonvulsant networks in the brain. (3) To determine whether anticonvulsant deep brain stimulation produces detrimental effects. And (4), to determine whether deep brain stimulation at the STN has an antiepileptogenic or neuroprotective effect. In the course of the proposed work, Dr. Lado will learn methods of intracranial injection, induction of chemical and electrical seizures, in vivo electrophysiology to record single unit and population activity, 2deoxyglucose autoradiography to map metabolic activation, neuroanatomical methods to detect synaptic reorganization and neuronal injury, and biostatistics. The proposed studies are designed to translate the insights and results obtained from animal research into improved treatments of human epilepsy. Moreover, the work described in this proposal will provide the investigator with an opportunity to acquire the necessary intellectual and technical skills be an independent investigator in translational i epilepsy research.

描述（申请人提供）：此申请适用于指导 弗雷德·拉多（Fred Lado），医学博士学位的临床科学家发展奖（K08）。拉多博士 在康奈尔大学医学上获得了神经病学临床培训 学院，随后在艾伯特·爱因斯坦医学院（Albert Einstein of Medicine）的脑电图和癫痫病中重点介绍了临床神经生理学研究金。他的 阿尔伯特神经病学系的现任教师任命 爱因斯坦医学院始于1999年。他以前获得了博士学位 他使用MagnetoencePaharography对人类受试者的调查，但他是 当前使用动物的体内实验方法学习，因为 最适合他的长期职业目标。他的目标是研究皮质下 调节和传播癫痫活性并发展的结构 针对这些区域的疗法以控制人类癫痫。这 阿尔伯特·爱因斯坦医学院和蒙特菲奥尔医学中心，主要 学院教学医院，在基本神经科学方面提供广泛的优势 和临床癫痫学。实验室空间，设备，办公空间和通道 对于跨多个学科的成熟科学家来说，很容易获得。 医疗也可以提供教学教学的机会 大学和专门的夏季课程。环境和支持 可供候选人使用，非常适合促进和培养博士。 拉多的长期专业目标。 Lado博士建议研究电气的抗惊厥作用 刺激下丘脑核。这些调查的目标是 四倍。 （1）研究使用深的新的对成年人癫痫发作的新对待 丘脑下核（STN）的脑电刺激。 （2）至 研究STN刺激的抗惊厥作用的机制 进一步发展我们对内在抗惊厥药的基本理解 大脑中的网络。 （3）确定抗惊厥的深脑是否 刺激会产生有害影响。 （4），确定是否深 STN处的脑刺激具有抗癫痫药或神经保护作用 影响。在拟议的工作过程中，Lado博士将学习 颅内注射，化学和电癫痫的诱导，体内 电生理记录单个单位和种群活动，2Deoxyglucose 放射自显影以绘制代谢激活，神经解剖学方法检测 突触重组和神经元损伤以及生物统计学。 拟议的研究旨在转化见解和结果 从动物研究中获得的改善人类癫痫的治疗方法。 此外，本提案中描述的工作将为调查员提供 有机会获得必要的智力和技术技能 成为转化I癫痫研究的独立研究者。