PHOSPHOCREATINE RECOVERY IN WOMEN W/ CHRONIC FATIGUE SYNDROME

患有慢性疲劳综合症的女性磷酸肌酸的恢复

• 批准号: 6657661
• 负责人: KEVIN MCCULLY
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6657661
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; congenital disorders; connective tissue; growth /development; infant mortality; liver; low birth weight infant human; magnetic resonance imaging; mother /infant health care; respiratory system; surgery; technology /technique

In Utero surgical techniques for the reversal of anatomical malformations are developed in small mammalian models. The design of new techniques suffers from lack of non-invasive pre- and post-surgical fetal monitoring. We have applied high resolution MRI to in-vivo, in-utero imaging of a rat model for Congenital Diaphragmatic Hernia (CDH). CDH is a developmental anomaly which involves incomplete closure of the diaphragm, herniation of the liver and abdominal viscera into the thoracic cavity, and lung hypoplasia. Eight dams were imaged on days 19-22 of gestation (once a day) to diagnose the presence or absence of CDH and monitor the effects of surgery. Those who were shown to be CDH+ on day 19 underwent immediate surgical tracheal ligation to reverse pulmonary hypoplasia and force the abdominal contents from the thoracic cavity. 39 rat fetuses were imaged using a multislice, T2 weighted, fast spin echo sequence on a 4T whole body imaging system (GE, Signa). Pathology and results of surgery were confirmed post-mortem by high resolution imaging (9.4T) and subsequent microscopic dissection. This information will help in the use and development of in utero intervention for treatment of congenital abnormalities.

逆转解剖结构的子宫内手术技术 畸形是在小型哺乳动物模型中产生的。 的设计 新技术缺乏非侵入性预处理和 术后胎儿监测。 我们应用了高分辨率 MRI 先天性大鼠模型的体内、子宫内成像 膈疝（CDH）。 CDH 是一种发育异常 涉及膈肌闭合不完全、肝疝 腹腔内脏进入胸腔，肺发育不全。 八只母坝在妊娠第 19-22 天进行了成像（每天一次），以 诊断 CDH 是否存在并监测其影响 外科手术。 第 19 天显示为 CDH+ 的患者接受了 立即进行气管结扎手术以逆转肺发育不全 并将腹腔内容物从胸腔中挤出。 39 老鼠 使用多层、T2 加权、快速自旋回波对胎儿进行成像 4T 全身成像系统（GE，Signa）上的序列。 病理学和 手术结果通过高分辨率尸检得到证实 成像（9.4T）和随后的显微解剖。 这 信息将有助于子宫内的使用和开发 干预治疗先天性异常。