MR NEAR INFRARED SPECTROSCOPY MUSCLE METABOLISM DURING RECOVERY EXERCISE: HUMANS

恢复运动期间的 MR 近红外光谱肌肉代谢：人类

• 批准号: 6121065
• 负责人: KEVIN MCCULLY
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121065
• 关键词: aging; biomedical resource; enzymes; female; human subject; musculoskeletal system; technology /technique

The purpose of this study was to develop new methodologies to measure muscle metabolism and blood flow in older humans, both with normal and reduced blood flow (peripheral vascular disease). Progress in this year was testing of a method using partial cuff ischemia during recovery from submaximal exercise. With MRS, we measure PCr recovery with a blood pressure cuff (positioned on the upper leg) inflated to 0, 50, 60, 70, and 90 mmHg. Similar measurements are made using duplex Doppler to determine the relative effect of the partial cuff pressures on muscle blood flow. NIRS measurements of oxygen saturation were collected during both MRS and Doppler measurements. Preliminary results suggest normal subjects have no impairment in PCr recovery with flow reductions up to 25%. With greater flow reductions, both flow and PCr recovery are impaired. Significant correlations were found between NIRS recovery and peak Doppler flow after ischemia in patients with mild PVD. Acceptab le levels of reproducibility suggest additional data will allow mathematical determination of relative contributions of blood flow and mitochondrial capacity in determining muscle metabolism in elderly healthy and diseased subjects.

本研究的目的是开发新的方法 测量老年人的肌肉代谢和血流量，两者均采用 血流量正常和减少（周围血管疾病）。 进步 今年正在测试一种使用部分袖带缺血的方法 从次最大运动恢复期间。 通过 MRS，我们测量 PCr 使用血压袖带进行恢复（位于大腿上） 充气至 0、50、60、70 和 90 mmHg。 进行了类似的测量 使用双工多普勒确定部分的相对效果 袖带压力对肌肉血流的影响。 氧气的 NIRS 测量 在 MRS 和多普勒测量期间收集饱和度。 初步结果表明正常受试者的 PCr 没有损害 流量减少高达 25% 的恢复。 流量更大 减少，流量和 PCR 恢复都会受到损害。 重要的 NIRS 恢复率与峰值多普勒血流之间存在相关性 轻度PVD患者缺血后。 可接受的水平 再现性表明额外的数据将允许数学 确定血流的相对贡献和 线粒体决定老年人肌肉代谢的能力 健康和患病受试者。