VIRAL VECTOR CORE FACILITY

病毒载体核心设施

• 批准号: 6633840
• 负责人: James S Norris
• 金额: $ 14.06万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6633840
• 关键词: Adenoviridae; Lentivirus; adeno associated virus group; biomedical facility; genetic transduction; helper virus; microorganism culture; recombinant virus; transfection /expression vector

DESCRIPTION: (Applicant's Description) This proposal requests support to establish a Viral Vector Core Facility to enhance basic and translational cancer research at the Medical University of South Carolina. The Facility will focus on three key areas: vector design, vector production, and quality control. The specific aims of this shared resource are: (1) To assist cancer center investigators in the selection and design of vectors to express genes of interest. (2) To provide services including creating recombinant adenovirus, and producing high-titer viral stocks; producing high titers of helper virus-free AAV vectors; establishing retroviral/lentiviral vector- producer cells, and generating and concentrating viral stocks for transduction; and providing quality control and standardized vectors for gene transfer studies. (3) To use this shared resource as a central facility for quality control and technical assistance to standardize the application of vectors. The facility will be directed by an established investigator with eight years of experience in vector development and in managing such a facility. Projects benefiting from this facility include steroid modulation of tumor cell growth, induction and analysis of murine prostate cancer, prostate, breast and brain cancer gene therapy, clinical phenotype targeted antifolate chemotherapy, oligonucleotides as inhibitors of DNA replication, T cell recognition of tumor antigen, tumor growth arrest mediated by transcription repressors, hormonal modulation of taxol action in solid tumors, 5- fluorouracil target in eukaryotic cells, inhibition of IGF mediated tumorigenesis, and molecular variables in fusion toxin efficacy. A detailed organizational plan, substantial institutional support, and a long term plan for maintenance ensure the sustained viability of the proposed Viral Vector Core Facility.

描述：（申请人的描述）该提案请求支持 建立病毒载体核心设施，增强基础和转化能力 南卡罗来纳医科大学的癌症研究。该设施将 专注于三个关键领域：矢量设计、矢量制作和质量 控制。该共享资源的具体目标是： (1) 帮助癌症 中心研究人员选择和设计表达基因的载体 的兴趣。 (2) 提供重组等服务 腺病毒，并生产高滴度病毒储液；产生高滴度 无辅助病毒的 AAV 载体；建立逆转录病毒/慢病毒载体- 生产细胞，并产生和浓缩病毒储备 转导；并提供基因质量控制和标准化载体 转学。 (3) 使用此共享资源作为中央设施 质量控制和技术援助，以标准化应用 向量。该设施将由一名已确定的调查员领导 在载体开发和管理此类载体方面拥有八年经验 设施。受益于该设施的项目包括类固醇调节 小鼠前列腺癌、前列腺癌的肿瘤细胞生长、诱导和分析 乳腺癌和脑癌基因治疗，临床表型靶向抗叶酸药物 化疗、寡核苷酸作为 DNA 复制抑制剂、T 细胞 识别肿瘤抗原，转录介导的肿瘤生长停滞 阻遏物，实体瘤中紫杉醇作用的激素调节，5- 氟尿嘧啶靶标真核细胞，抑制 IGF 介导 肿瘤发生和融合毒素功效的分子变量。详细的 组织计划、实质性机构支持和长期计划 用于维护，确保拟议病毒载体的持续活力 核心设施。