CORE--TISSUE CULTURE AND MONOCLONAL ANTIBODIES

核心——组织培养和单克隆抗体

• 批准号: 6650623
• 负责人: MARY L. MICHAELIS
• 金额: $ 16.03万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6650623
• 关键词: biomedical facility; mental retardation; molecular pathology; monoclonal antibody; tissue /cell culture

Investigations into the cellular and molecular mechanisms that lead to abnormalities associated with developmental disabilities and mental retardation are increasingly being carried out with in vitro model systems such as cell cultures, including cells transfected with foreign genes and cells derived from transgenic and knockout mice. Advances in understanding the mechanisms for the biochemical pathology of diseases that lead to mental retardation, such as Down syndrome or the mucopolysaccharidoses, have been critically dependent upon the development of techniques for studying the altered cellular and biochemical milieu in isolated cell culture systems. In addition, state-of- the-art research in developmental biology frequently requires that investigators develop unique biological reagents such as polyclonal and monoclonal antibodies, various types of nucleotide probes, and constructs for transfection of foreign genes into cells. The Tissue Culture/Monoclonal Antibodies Laboratory (TCMAC) has facilitated the research of several of the Kansas MRDDRC investigators by providing core facilities and services that enable them to develop, maintain, and characterize numerous cellular model systems for exploring aspects of normal cell growth, signaling pathways that regulate cell viability, and effects of exogenous agents that enhance or disrupt cell growth. The TCMAC Laboratory takes great pride in the many enhancements that have been made in the facility during the past grant period as these improvements enable the lab to provide the highest level of support for MRDDRC investigators. The facility has also successfully developed unique polyclonal and monoclonal antibodies that served the needs of individual investigators. The antibody work done for Robert Palazzo has provided an excellent tool in his efforts to identify critical proteins in the centrosome and monitor changes during the cell cycle. The series of antibodies developed for Elias Michaelis have led to important discoveries regarding protein expression and localization in response to chronic ethanol exposure. Antibodies developed by the facility during the past grant period are currently being utilized at other universities in collaborative studies, and a major company has expressed interested in marketing some of those highly specific and valuable tools so they will be available to scientists on an international level. As the TCMAC looks to the future and means of enhancing the Center's focus on biobehavioral processes, the Core will play an expanding role in studies involving gene transfections, growth of ES cells, and other techniques required to produce transgenic or knockout mice. The TCMAC has been and continues to be a relatively small core, but it has played an extremely important role in attracting new, highly productive scientists to the Center itself and encouraging them to apply their talents to new, highly productive scientists to the Center itself and encouraging them to apply their talents to question related to developmental disabilities. For example, the TCMAC was instrumental in the attracting Rick Dobrowsky, Doug Ruden and Robert Palazzo to the MRDDRC shortly after they joined to KU faculty. This core is likely to continue in this role as the University hires new faculty members in the areas of molecular genetics, bioinformatics and developmental neurobiology. The goal of the TCMAC has been and will continue to be the provision of unique bioreagents, skilled technical support, and state-of-the-art equipment to facilitate and enhance the research of MRDDRC investigators who study molecular events leading to developmental disabilities. The TCMAC will continue to facilitate MRDDRC research at The University of Kansas by: (1) Maintaining a centralized facility that provides a cost-effective alternative to purchase of expensive equipment and services by MRDDRC investigators. (2) Producing and characterizing unique monoclonal/polyclonal antibodies and maintaining hybridoma cell lines for projects requiring a consistent supply of antibodies.

越来越多地使用诸如细胞培养物的体外模型系统（包括用异物基因和转基因和基因敲除小鼠衍生的细胞）进行对导致与发育障碍和智力降低相关的异常的细胞和分子机制的研究。理解导致智力低下的疾病生化病理学的机制，例如唐氏综合症或粘多糖糖果，已严重取决于在孤立的细胞培养系统中研究改变细胞和生化环境的技术的发展。此外，在发育生物学方面的最新研究经常要求研究人员开发独特的生物试剂，例如多克隆和单克隆抗体，各种类型的核苷酸探针，以及将外源基因转染到细胞中的构造。 The Tissue Culture/Monoclonal Antibodies Laboratory (TCMAC) has facilitated the research of several of the Kansas MRDDRC investigators by providing core facilities and services that enable them to develop, maintain, and characterize numerous cellular model systems for exploring aspects of normal cell growth, signaling pathways that regulate cell viability, and effects of exogenous agents that enhance or disrupt cell growth. TCMAC实验室对在过去的赠款期间在设施中进行的许多增强功能感到自豪，因为这些改进使实验室能够为MRDDRC调查人员提供最高水平的支持。该设施还成功地开发了针对个别研究人员需求的独特多克隆和单克隆抗体。罗伯特·帕拉佐（Robert Palazzo）进行的抗体工作为识别中心体中的关键蛋白质并在细胞周期内监测变化的努力提供了一种极好的工具。针对Elias Michaelis开发的一系列抗体导致了有关响应慢性乙醇暴露的蛋白质表达和定位的重要发现。该设施在过去的赠款期间开发的抗体目前正在其他大学中用于协作研究，一家主要公司表示有兴趣营销其中一些高度特定和有价值的工具，因此在国际层面上可以向科学家使用它们。随着TCMAC着眼于未来以及增强中心对生物行为过程的关注的手段，该核心将在涉及基因转染，ES细胞的生长以及产生转基因或基因敲除小鼠的研究中发挥不断的作用。 TCMAC曾经是并且继续是一个相对较小的核心，但是它在吸引新的，高产的科学家到中心本身中发挥了极为重要的作用，并鼓励他们将自己的才能应用于新的，高产的科学家对中心本身，并鼓励他们将其才华应用于与发育障碍有关的问题。例如，TCMAC在吸引Rick Dobrowsky，Doug Ruden和Robert Palazzo加入MRDDRC之后不久就加入了KU教职员工。随着大学聘请分子遗传学，生物信息学和发育神经生物学领域的新教师，该核心很可能会继续担任这一角色。 TCMAC的目标一直是并将继续是提供独特的生物因素，熟练的技术支持和最先进的设备，以促进和增强研究导致发育障碍的分子事件的MRDDRC研究人员的研究。 TCMAC将继续在堪萨斯大学促进MRDDRC研究，并通过：（1）维护一个集中式设施，该设施为MRDDRC调查人员购买昂贵的设备和服务提供了具有成本效益的替代方案。 （2）产生和表征独特的单克隆/多克隆抗体，并维持需要一致抗体供应的项目的杂交瘤细胞系。