CORE--ANIMAL RESOURCE

核心--动物资源

• 批准号: 6663393
• 负责人: Bruce R Ksander
• 金额: $ 13.47万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663393
• 关键词: Chelonia; Primates; animal breeding; animal care; animal colony; biomedical facility; eye; ferrets; fish; guinea pigs; hamsters; veterinary science; vision

Abstract: Funds are requested by 30 vision scientists to support 4 research modules that facilitate and enhance interaction among the vision scientists in the various disciplines represented at the Schepens Eye Research Institute. The modules will extend endeavors of individual research programs by providing collaborative opportunities for projects in which investigators do not have expertise, funding, or technical capabilities. The modules are: Morphology, Animal Resource, Laboratory Computer Applications and Flow Cytometry. The Morphology Module will provide light and electron microscopy, confocal microscopy and image analysis capabilities and tissue preparation for histochemistry and in situ hybridization, thereby increasing of morphologic techniques to biochemists, pharmacologists, cell and molecular biologists, and immunologists who need correlative morphologic data for their research. The module houses and maintains shared equipment for morphologic work and is available to all members of the Core. The Animal Resource Module will provide expertise and assistance in animal surgical techniques and postoperative care, administration of anesthesia, drugs, and medications. In addition, it will also provide for daily animal husbandry that is required to maintain our high standards for animal housing, sanitation, and veterinary care. The Laboratory Computer Applications Module will provide support to individual and collaborative efforts by assisting in interfacing of microcomputers with lab equipment and by developing software for gathering, processing, and analyzing experimental data by microcomputer systems. The module will also provide assistance to projects involving image processing and analysis from several types of equipment sources. The module interacts actively with the Morphology and Flow Cytometry Modules supporting computer- based technology. The Flow Cytometry Module is a centralized service providing principle investigators and collaborators access to equipment and technical support for flow cytometry and cell sorting. Available to the investigators are the Coulter Epics XL flow cytometer and the Coulter ELITE EPICS ESP fluorescent cell sorting. Available to the investigators are the Coulter Epics XL flow cytometer and the Coulter ELITE EPICS ESP fluorescent cell sorter. The modules are staffed by personnel with specialized training in the respective fields and equipment. Each module is under the immediate supervision of an established, experienced investigator(s). The module heads constitute the Core Grant Committee, which is responsible to the Director of Research.

摘要： 30 名视觉科学家请求资金支持 4 个研究模块，这些模块促进和加强舍彭斯眼科研究所代表的各个学科的视觉科学家之间的互动。这些模块将为研究人员不具备专业知识、资金或技术能力的项目提供合作机会，从而扩展个别研究项目的努力。这些模块包括：形态学、动物资源、实验室计算机应用和流式细胞术。形态学模块将为组织化学和原位杂交提供光学和电子显微镜、共焦显微镜和图像分析功能以及组织制备，从而为需要相关形态学数据的生物化学家、药理学家、细胞和分子生物学家以及免疫学家提供更多的形态学技术。研究。该模块容纳并维护用于形态学工作的共享设备，并且可供核心的所有成员使用。动物资源模块将提供动物手术技术和术后护理、麻醉管理、药物和药物方面的专业知识和帮助。此外，它还将提供维持动物饲养、卫生和兽医护理高标准所需的日常畜牧业。实验室计算机应用模块将通过协助微型计算机与实验室设备的接口以及开发用于通过微型计算机系统收集、处理和分析实验数据的软件，为个人和协作努力提供支持。该模块还将为涉及多种类型设备源的图像处理和分析的项目提供帮助。该模块与支持计算机技术的形态学和流式细胞术模块积极交互。流式细胞术模块是一项集中服务，为主要研究人员和合作者提供流式细胞术和细胞分选的设备和技术支持。研究人员可以使用 Coulter Epics XL 流式细胞仪和 Coulter ELITE EPICS ESP 荧光细胞分选仪。研究人员可以使用 Coulter Epics XL 流式细胞仪和 Coulter ELITE EPICS ESP 荧光细胞分选仪。这些模块的人员均接受过各自领域和设备方面的专门培训。每个模块都受到一位资深的、经验丰富的调查员的直接监督。模块负责人组成核心资助委员会，该委员会对研究主任负责。