Microarray Analysis-Intracellular Infection/Autoimmunty

微阵列分析-细胞内感染/自身免疫

• 批准号: 6314829
• 负责人: MICHAEL G HUMPHREYS-BEHER
• 金额: $ 1.9万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314829
• 关键词: NOD mouse; clinical research; diabetes mellitus; enzyme linked immunosorbent assay; epidermal growth factor; growth factor; histology; homeostasis; human subject; insulin; insulinlike growth factor; medical complication; neurotrophic factors; oral health; periodontium disorder; radioimmunoassay; saliva; serum; transforming growth factors; wound healing

DESCRIPTION: The salivary glands are a major source of several factors, which play important roles in both oral and systemic organ homeostasis and wound healing. The roles of these factors, which include IGF-I, IGF-II, NGF, TGF alpha and beta, and EGF, has been well-studied. However, the fact that the salivary glands appear to be a major source of these growth factors present a interesting question both in the primary route of reentry into the system and the relative importance of these salivary- derived proteins systemically. In diabetic patients both (Type I and II) a major disease complication is diminished capacity of wound healing and in the oral cavity increased periodontal disease. A similar picture occurs in animal models of diabetes in there is also a progressive loss of growth factors from saliva in accordance with diabetes onset. Therefore, the investigators propose to look for the potential loss of salivary-derived growth factors associated with the observed decrease in wound healing capacity in diabetic patients. To accomplish this goal, they first plan to use the diabetic patient base of the University of Florida to investigate the changes in growth factor levels in patient saliva and serum after surgical procedures, as compared to healthy non-diabetic individuals undergoing similar procedures. Second, they intend to determine the influence of changes in growth factor levels in saliva on wound healing in the NOD mouse model for IDDM. With this, they intend to establish the NOD mouse as a viable model for this aspect of the disease and then to be able to employ this model to more thoroughly investigate the impact of decreased levels of salivary growth factors on experimentally introduced soft and hard tissue injuries. The results of these studies should elucidate the importance of salivary-derived growth factors on systemic homeostasis and wound repair and potentially provide insight into the viability of replacement strategies to combat several complications of human diabetes which may involve an underlying deficiency in wound repair mechanisms.

描述：唾液腺是多种因素的主要来源， 在口腔和全身器官稳态中发挥重要作用 伤口愈合。这些因素的作用，包括 IGF-I、IGF-II、 NGF、TGF α 和 β 以及 EGF 已得到充分研究。然而，事实 唾液腺似乎是这些生长的主要来源 因素在主要途径中提出了一个有趣的问题 再进入系统以及这些唾液的相对重要性 系统地衍生蛋白质。糖尿病患者（I 型和 II 型） 一个主要的疾病并发症是伤口愈合能力下降和 在口腔中增加牙周病。类似的画面也出现了 在糖尿病动物模型中，生长也逐渐丧失 唾液中的因素与糖尿病发病一致。因此， 研究人员建议寻找唾液来源的潜在损失 与观察到的伤口愈合下降相关的生长因子 糖尿病患者的能力。为了实现这个目标，他们首先计划 利用佛罗里达大学糖尿病患者基地进行调查 患者唾液和血清中生长因子水平的变化 与健康的非糖尿病个体相比，手术程序 正在经历类似的程序。其次，他们打算确定 唾液中生长因子水平的变化对伤口愈合的影响 IDDM 的 NOD 小鼠模型。由此，他们打算建立NOD 小鼠作为该疾病这方面的可行模型，然后能够 采用该模型更彻底地研究 实验引入的唾液生长因子水平降低 软组织和硬组织损伤。这些研究的结果应该 阐明唾液源性生长因子对全身系统的重要性 稳态和伤口修复，并有可能提供对 替代策略应对多种并发症的可行性 人类糖尿病可能涉及伤口修复的潜在缺陷 机制。