REGULATION OF ACINAR CELL PROLIFERATION

腺泡细胞增殖的调节

• 批准号: 2389418
• 负责人: MICHAEL G HUMPHREYS-BEHER
• 金额: $ 19.12万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2389418
• 关键词: acinar cell; active sites; antisense nucleic acid; calmodulin dependent protein kinase; cell growth regulation; cell proliferation; enzyme mechanism; epidermal growth factor; galactosyltransferases; gene expression; genetic library; genetic regulatory element; isoproterenol; laboratory mouse; laboratory rat; molecular cloning; parotid gland; protein tyrosine kinase; reporter genes; site directed mutagenesis; transcription factor; transfection

DESCRIPTION (Adapted from the investigator's Abstract): The present proposal continues the ongoing efforts of this investigator to elucidate the biological effects of beta 1,4-galactosyltransferase (Gal Tase), and the expression of GTA-K on acinar cell growth response. The following specific aims have been proposed: (1) Elucidate the chromosomal structure of the GTA-K and determine the role of the regulatory sequences in the expression of this protein. (2) To study the biological effects of the GTA-K through site-directed mutagenesis of various functional domains. It is anticipated that these studies will provide new information about one possible mechanism involved in the regulation of acinar cell growth. A model which has been widely used, for almost three decades, to study acinar cell growth is the acinar cell proliferation in response to beta adrenergic stimulation. This study is aimed towards further characterizing the mechanisms associated with this proliferative event.

描述（改编自研究者的摘要）：目前 该提案继续了该调查员为阐明 β1,4-半乳糖基转移酶（Gal Tase）的生物学效应，以及 GTA-K 的表达对腺泡细胞生长反应的影响。 具体如下 提出的目标是：（1）阐明染色体结构 GTA-K 并确定调控序列在表达中的作用 这种蛋白质。 (2) 通过研究GTA-K的生物学效应 各种功能域的定点诱变。 预计 这些研究将提供有关一种可能机制的新信息 参与腺泡细胞生长的调节。 一个模型已经被 近三十年来，广泛用于研究腺泡细胞生长的是 腺泡细胞响应β肾上腺素能刺激而增殖。 这 研究的目的是进一步描述与相关的机制 这个扩散事件。