Core--Myocyte

核心--肌细胞

• 批准号: 6654190
• 负责人: ATSUKO YATANI
• 金额: $ 29.64万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6654190
• 关键词: animal tissue; biomedical facility; cardiac myocytes; electrophysiology; tissue /cell preparation

This project will provide: 1) Preparation of enzymatically isolated myocytes from pig. Myocytes from other animals such as woodchuck, rat and mouse will also be prepared as required by the individual projects. 2) Characterization of isolated myocyte size. 3) Characterization of myocyte mechanics (contractile parameters) and intracellular Ca2+ transients. 4) Characterization of basic electrophysiological properties including action potentials and membrane currents. 5) Maintenance of equipment including three Langendorff apparatus for myocyte isolation, three video motion edge detectors, two fluorescent photomoters, and two patch-clamp recording systems. 6) Isolation of trabecular muscle.

该项目将提供： 1）从猪中制备酶法分离的肌细胞。来自其他动物（如土拨鼠、大鼠和小鼠）的肌细胞也将根据各个项目的要求进行制备。 2) 分离的肌细胞大小的表征。 3) 肌细胞力学（收缩参数）和细胞内 Ca2+ 瞬变的表征。 4) 基本电生理特性的表征，包括动作电位和膜电流。 5) 设备维护，包括三个用于心肌细胞分离的 Langendorff 装置、三个视频运动边缘检测器、两个荧光光电机和两个膜片钳记录系统。 6）小梁肌的分离。