Bacterial genes & proteins involved in redox transform

细菌基因

• 批准号: 6667491
• 负责人: Bradley M. Tebo
• 金额: $ 17.5万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667491
• 关键词: Bacillus subtilis; Escherichia coli; bacteria; bacterial genetics; bacterial proteins; biotransformation; chromium; copper; environmental toxicology; gene expression; ground water; hazardous substances; manganese; metalloenzyme; metals; oxidation reduction reaction; water pollution

The objective of this project will be to study the mechanisms of transformation and fate of toxic substances primarily in coastal environments. This project will focus on understanding the molecular mechanisms by which metal contaminants are transformed from soluble phases to insoluble phases and to evaluate the contribution from different physiological groups of bacteria in this process. Experiments will center on re-expression of genes and characterization of multi-copper oxidase- like proteins involved in catalyzing Mn (II) oxidation in different bacterial groups. In addition Dr. Tebo has identified several novel bacteria that resist the toxic levels of Cr(VI) and grow with Cr(VI) as an electron acceptor. The second part of his project will examine the biochemical basis for Cr(VI) reduction in these novel Cr(VI)-reducing bacterial and study the regulation of Cr(VI) and the expression of other genes in response to Cr(VI).

该项目的目的是研究主要在沿海环境中的有毒物质转化和命运的机制。该项目将着重于理解金属污染物从可溶相变成不溶性相的分子机制，并在此过程中评估不同生理细菌的贡献。实验将集中于重新表达基因的表达以及在不同细菌组中催化Mn（II）氧化的多型氧化酶类似蛋白的表征。另外，Tebo博士确定了几种抗CR（VI）毒性水平并以CR（VI）作为电子受体生长的新型细菌。他项目的第二部分将检查这些新型Cr（VI）还原细菌的Cr（VI）降低的生化基础，并研究CR（VI）的调节以及其他基因对CR（VI）的表达。