Human Genome Array: Technology for Functional Analysis

人类基因组阵列：功能分析技术

• 批准号: 6597155
• 负责人: MICHAEL P. SNYDER
• 金额: $ 11.24万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6597155
• 关键词: DNA binding protein; DNA replication; DNA replication origin; binding sites; biotechnology; chromosomes; functional /structural genomics; gene mutation; genetic mapping; genetic polymorphism; genetic techniques; human genetic material tag; human subject; immunoprecipitation; microarray technology; molecular biology information system; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; technology /technique development; transcription factor

DESCRIPTION: (provided by applicant) We propose to establish a center to build genomic DNA arrays and develop novel technologies that will use these arrays for the large-scale functional analysis of the human genome. 0.3-1.4 kb fragments of nonrepetitive DNA from each of chromosomes 22, 21, 20, 19,7, 17, and perhaps the X chromosome will be prepared by PCR and attached to microscope slides. The arrays will be used to develop technologies for the large-scale mapping of 1) Transcribed sequences. 2) Binding sites of chromosomal proteins 3) Origins of replication. 4) Genetic mutation and variation. A web-accessible database will be constructed to house the information generated in this study; data from other studies will also be integrated into the database. The arrays and technologies will be made available throughout both the Yale University and the larger scientific community. They will be integrated into our training programs for postdoctoral fellows, graduate students and undergraduates at Yale. We expect these procedures to be applicable to the analysis of the entire human genome and the genomes of many other organisms.

描述：（由申请人提供）我们建议建立一个中心来建设 基因组 DNA 阵列并开发使用这些阵列的新技术 用于人类基因组的大规模功能分析。 0.3-1.4 kb 来自每条染色体 22、21、20、19,7、17 的非重复 DNA 片段， 也许 X 染色体将通过 PCR 制备并连接到显微镜上 幻灯片。这些阵列将用于开发大规模技术 1) 转录序列的映射。 2) 染色体蛋白的结合位点 3）复制的起源。 4）基因突变和变异。一个可以通过网络访问的 将建立数据库来存放本研究中产生的信息； 其他研究的数据也将被整合到数据库中。 这些阵列和技术将在耶鲁大学和 大学和更大的科学界。他们将被整合到 我们为博士后研究员、研究生和 耶鲁大学本科生。我们希望这些程序适用于 分析整个人类基因组和许多其他生物体的基因组。