Search for a Hypertensive Renal Failure Gene

寻找高血压肾衰竭基因

• 批准号: 6487537
• 负责人: Michael Ignatius Jensen-Seaman
• 金额: $ 3.83万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6487537
• 关键词: gene mutation; genetic susceptibility; genetics; introns; laboratory rat; nucleic acid sequence; renal hypertension

DESCRIPTION: (provided by applicant): End-stage renal disease (ESRD) remains a major health problem in the United States and continues to increase annually. By 1997 there were over 300,000 patients enrolled in the Medicare-fund ESRD program for renal replacement therapy, will the total cost of care for ESRD patients in the United States at over $15 billion annually. The goal of this project is to search for a gene (or genes) that confers susceptibility to hypertensive ESRD in a rat model. A region of rat chromosome 1 has previously been identified as harboring a gene for renal failure (termed Rf-1), from a backcross-that has been replicated in an F2 intercross, both derived from the Fawn Hooded Hypertensive (FHH) rat and the normotensive ACI parental strains of rat. Under the proposed project I will search for this gene using a variety of state of the art molecular genetics techniques designed to narrow down the universe of possible disease-causing genes; ultimately the gene will be sequenced in both the disease strain (FHH) and normal strains (ACI, Brown Norway) to identify molecular changes potentially responsible for the susceptibility to hypertensive ESRD. As it is believed that the mechanisms of renal disease onset and progression are the same or similar in humans as in rats, this work may ultimately lead to a better understanding of ESRD in humans.

描述：（申请人提供）：终末期肾脏疾病（ESRD）仍然是 美国的主要健康问题，每年继续增加。 到1997年，有30万名患者参加了Medicare基金ESRD 肾脏替代疗法计划，ESRD的总护理费用 美国的患者每年以超过150亿美元的价格。目标的目标 项目是搜索赋予对易感性的基因（或基因） 大鼠模型中的高血压ESRD。大鼠染色体1的区域以前具有 被确定为具有肾衰竭基因（称为RF-1） 反向交叉 - 已在F2中复制，这两者都来自 小鹿连帽高血压（FHH）大鼠和正常的ACI父母菌株 鼠。在拟议的项目下，我将使用各种 旨在缩小范围的最先进的分子遗传学技术 可能引起疾病的基因的宇宙；最终，基因将是 在疾病菌株（FHH）和正常菌株中测序（ACI，棕色 挪威），以确定可能造成的分子变化 高血压ESRD的敏感性。据信， 肾脏疾病的发作和进展与人类相同或相似 老鼠，这项工作最终可能会更好地理解ESRD 人类。