SOCIAL AND NEUROENDOCRINE REGULATION OF THE PERIOD GENE

周期基因的社会和神经内分泌调节

• 批准号: 6498806
• 负责人: GENE E ROBINSON
• 金额: $ 35.43万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6498806
• 关键词: Hymenoptera; RNase protection assay; animal developmental psychology; circadian rhythms; gene expression; genetic library; genetic manipulation; genetic regulation; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; juvenile hormones; messenger RNA; neuroendocrine system; neuroregulation; northern blottings; nucleic acid sequence; nucleic acid structure; octopamine; polymerase chain reaction; radioimmunoassay; social behavior; southern blotting; western blottings

Molecules that regulate circadian rhythmicity are also thought to influence the functioning of processes at different temporal scales. The goal of this project is to test an important prediction of this model: given that behavioral and molecular data suggest an association between behavioral maturation and behavioral circadian rhythms in the honey bee, then social and neuroendocrine factors known to influence rate of behavioral maturation should also influence per expression. The following six lines of inquiry will be pursued. 1. Characterize the bee per gene: We have generated a full-length cDNA sequence and also have partially sequenced a genomic clone and will do comparative analyses (with H. Robertson). 2. Determine whether per mRNA levels in the bee brain change during behavioral maturation. Young bees, which work in the hive, have no behavioral circadian rhythms while older bees, which forage on flowers, do; per mRNA levels in the brain change in association with this behavioral maturation. This correlative analysis provides baseline data for the treatment experiments in Aims 4-6. 3. Localize and measure levels of brain PER. Preliminary immunocytochemical results indicate that maturational changes in per mRNA are reflected at the protein level. Continued analyses will be coupled with in situ hybridization to provide additional confirmation (with S. Fahrbach and K. Siwicki). Results of this correlative study will also be used to provide a baseline for Aims 4-6. 4. Determine whether changes in social environment, known to influence behavioral maturation, also influence per expression in the brain. We will take full advantage of a uniquely powerful set of behavioral manipulations to dissociate chronological age, stage of behavioral maturation, and experience, all while working with bees that are highly related to each other. Preliminary results support this hypothesis. 5. Determine whether juvenile hormone and octopamine manipulations, known to influence behavioral maturation, cause changes in both rhythmic locomotor activity and 6. per expression. Preliminary results for locomotor activity support this hypothesis. We hypothesize that juvenile hormone analog and octopamine treatments will increase per expression while allatectomy and octopamine antagonist treatment will decrease it. The principal significance of this research is that it attempts to experimentally determine for the first time whether the expression of a clock gene is dependent upon a broader array of influences than those strictly associated with circadian rhythmicity.

调节昼夜节律的分子也被认为会影响不同时间尺度下过程的功能。 该项目的目的是测试该模型的重要预测：鉴于行为和分子数据表明，行为成熟与蜂蜜蜜蜂中的行为昼夜节律之间存在关联，而已知会影响行为成熟速率的社会和神经内分泌因子也应影响每个表达。 将进行以下六行调查。 1。表征每个基因的蜜蜂：我们已经产生了一个全长的cDNA序列，并且已经部分测序了基因组克隆，并将进行比较分析（与H. Robertson）。 2。确定行为成熟过程中蜜蜂脑中的每个mRNA水平是否变化。在蜂巢中起作用的年轻蜜蜂没有行为昼夜节律，而较大的蜜蜂则在花上觅食。与这种行为成熟相关的大脑变化中的每个mRNA水平。 该相关分析为AIM 4-6中的治疗实验提供了基线数据。 3。局部和测量大脑的水平。 初步免疫细胞化学结果表明，每个mRNA的成熟变化反映在蛋白质水平上。 持续的分析将与原位杂交相结合以提供额外的确认（S. fahrbach和K. siwicki）。 这项相关研究的结果也将用于为目标4-6提供基线。 4。确定社会环境的变化是否已知会影响行为成熟，也会影响大脑中的每个表达。 我们将充分利用一组独特的行为操纵集，以分离时间顺序的年龄，行为成熟的阶段和经验，同时与彼此高度相关的蜜蜂合作。 初步结果支持这一假设。 5。确定已知会影响行为成熟的少年激素和章鱼胺的操纵是否会导致节奏运动活性的变化和每表达6。。 运动活动的初步结果支持这一假设。 我们假设少年激素类似物和章鱼胺治疗将增加每个表达，而allat抗切除术和章鱼拮抗剂的治疗将降低它。 这项研究的主要意义在于，它试图首次确定时钟基因的表达是否取决于比与昼夜节律严格相关的影响更广泛的影响。