MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 6509503
• 负责人: Jonathan J. Wisco
• 金额: $ 2.46万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6509503
• 关键词: Macaca mulatta; age difference; aging; behavioral /social science research tag; bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain mapping; brain metabolism; cognition; hypertension; magnetic resonance imaging; morphometry; neurosurgery; nuclear magnetic resonance spectroscopy

In a non-human primate model for aging and cerebrovascular disease, the interactive effect of normal aging and induced systolic hypertension will be investigated. This investigation will employ clinically available neuroimaging techniques to examine the brains of young and middle-aged rhesus monkeys (Macaca mulatta) in vivo. Magnetic Resonance Imaging (MRI) and Magnetic Resonance Spectroscopy (MRS) will be utilized to explore morphologic and metabolic changes, respectively, in the young and middle-aged cohorts. Behavioral tests based on clinically applied neuropsychological measures will determine cognitive ability as well. Following completion of behavioral testing, a surgical procedure to coarct the thoracic aorta will induce systolic hypertension in both cohorts. Post-op examination of the cohorts' brains will be accomplished using methods identical to pre-op procedures-morphological examination using MRI and MRS, and cognitive ability measurement using behavioral tests. Further examination of these brains will be accomplished post- mortem, as soon as behavioral tests are completed. Brain tissue will be stained to reveal morphology, metabolic activity, and microglia reactivity, and will be quantified using optical sampling and stereological methodology. Finally, this investigation will conclude by comparing brain morphology, metabolic activity and cognitive ability between the young and middle-aged cohorts pre- and post-operatively.

在非人类衰老和脑血管疾病的非人类灵长类动物模型中，将研究正常衰老和诱导的收缩压高血压的相互作用。 该研究将采用临床上可用的神经影像学技术来检查体内年轻和中年恒河猴（Macaca Mulatta）的大脑。 磁共振成像（MRI）和磁共振光谱（MRS）将分别用于探索年轻和中年同伙中的形态和代谢变化。 基于临床应用神经心理学措施的行为测试也将决定认知能力。 在完成行为测试后，胸腔主动脉的手术程序将在两个队列中诱导收缩压。 在使用MRI和MRS和使用行为测试的认知能力测量的方法中，将使用与OP前手术相同的方法检查人群的大脑检查后检查。一旦完成行为测试，将立即完成这些大脑的进一步检查。 脑组织将被染色以揭示形态学，代谢活性和小胶质细胞反应性，并将使用光学采样和立体方法进行量化。 最后，这项研究将通过比较术前和术后年轻人群和中年同龄人之间的脑形态，代谢活性和认知能力来结束。