Single Molecule Studies of Unimolecular Nanocarriers

单分子纳米载体的单分子研究

• 批准号: 6550642
• 负责人: LAWRENCE William JOHNSON
• 金额: $ 5.23万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-03 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6550642
• 关键词: amphiphilicity; cell membrane; confocal scanning microscopy; drug delivery systems; fluorescent dye /probe; liposomes; membrane permeability; microcapsule; nanotechnology; pharmacokinetics; polymers; solubility; spectrometry

DESCRIPTION (provided by applicant): Single molecule spectroscopic techniques will be used to assay the physical properties of controlled drug delivery systems, unimolecular amphiphilic nanocarriers (UAN). We will relate the physical properties of the UAN's polymer core to its ability to solubilize and deliver drug molecules. The specific aims of this project are to understand the encapsulation process that protects and solubilizes the drug molecule, and to determine the process of distribution and delivery of the drug molecule; a UAN can be used not only to solubilize a hydrophobic drug molecule, but also to protect it from environmental degradation. These studies will also help determine what factors will enable the UAN to better deliver the drug through cell membranes. The principle techniques in studying encapsulation, will be environmentally sensitive bulk and single molecule time resolved spectroscopy. A series of UAN's with modified polymer cores will be used to determine how the different flexibilities of the polymer effect solubilization of a fluorescent probe molecule. To assess the ability of a UAN to preferentially deliver drugs by permeating a membrane, actual delivery of bioactive molecules to liposomes will be dynamically imaged using scanning confocal microscopy and single molecule detection.

描述（由申请人提供）：单分子光谱技术将用于测定受控药物输送系统的物理特性，单分子两亲性纳米载体（UAN）。我们将将UAN聚合物核的物理特性与其溶解和传递药物分子的能力联系起来。该项目的具体目的是了解保护和溶解药物分子的封装过程，并确定药物分子的分布和递送过程； UAN不仅可以用来溶解疏水性药物分子，还可以保护其免受环境降解。这些研究还将有助于确定哪些因素将使UAN通过细胞膜更好地输送药物。研究封装的主要技术将是环境敏感的大量和单分子时间分辨光谱法。一系列具有改性聚合物核的UAN将用于确定荧光探针分子的聚合物效应溶解的不同屈曲度。为了评估UAN通过渗透膜优先输送药物的能力，将使用扫描共聚焦显微镜和单个分子检测来动态成像生物活性分子向脂质体的实际递送。