Microfluidic Systems for the Applied Life Sciences

适用于应用生命科学的微流体系统

• 批准号: 6520572
• 负责人: James D Sterling
• 金额: $ 14.15万
• 依托单位: KECK GRADUATE INST OF APPLIED LIFE SCIS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520572
• 关键词: bioengineering /biomedical engineering; capillary electrophoresis; computer program /software; electrical property; fluid flow; fluorescence spectrometry; fluorescent dye /probe; gene expression; high throughput technology; hydropathy; microarray technology; microprocessor /microchip; nanotechnology; nonblood rheology; nucleic acid purification; physical separation; polymerase chain reaction; sample collection; surface property; technology /technique development

DESCRIPTION (provided by applicant): Interdisciplinary activity is an integral part of modern biotechnological research and development efforts. In an attempt to engender interdisciplinary work involving investigators with quantitative scientific and engineering backgrounds, the NIH has solicited applications for the Mentored Quantitative Research Career Development Award. In response, this grant application proposes a career development plan for the Principal Investigator, Dr. James D. Sterling, involving both didactic and research development components. The plan will guide his career transition from mechanical engineering in combustion and aerospace applications to an independent research career in quantitative bioengineering for the applied life sciences. This application describes the combination of formal lectures at the Keck Graduate Institute of Applied Life Sciences, short courses at UCLA, professional conferences, and informal study that will be used to develop the necessary background in molecular biology and genomic and proteomic sciences. In addition, the planned research activity involving fundamental microfluidics process characterization and system-level design is presented. During the first several years, characterization of fundamental microfluidic phenomena as componentry for biochips will be performed. This will involve the development of various flow control methods and molecular separation methods. As this research progresses, the P1 and mentors will identify a focus area in which to develop a systems-level design for high-throughput biological sample processing, analysis and/or archiving. Thus, the P1 and the Mentors will share some resources and will work closely to ensure that the microfluidics research is performed in support of high-throughput gene/protein expression analysis. This plan provides a phased development effort that will prepare Dr. Sterling to compete for NIH support as an independent researcher or as a leading member of a multi-disciplinary research team.

描述（由申请人提供）：跨学科活动是一个不可或缺的部分 现代生物技术研究和开发工作的一部分。在一次尝试中 开展涉及定量研究的跨学科工作 科学和工程背景，NIH 已征集申请 指导定量研究职业发展奖。作为回应，这 拨款申请为校长提出职业发展计划 研究员 James D. Sterling 博士，涉及教学和研究 开发组件。 该计划将指导他从机械工程专业过渡到 燃烧和航空航天应用到独立研究生涯 应用生命科学的定量生物工程。这个应用程序 描述了凯克研究生院正式讲座的结合 应用生命科学、加州大学洛杉矶分校的短期课程、专业会议和 非正式研究将用于开发必要的背景 分子生物学以及基因组和蛋白质组科学。 此外，计划的研究活动涉及基础微流体学 提出了过程表征和系统级设计。第一次期间 几年来，基本微流体现象的表征为 将进行生物芯片的元件制造。这将涉及到开发 各种流量控制方法和分子分离方法。正如这个 随着研究进展，P1 和导师将确定一个重点领域 开发高通量生物样品的系统级设计 处理、分析和/或归档。因此，P1 和导师将分享 一些资源并将密切合作以确保微流体研究 进行以支持高通量基因/蛋白质表达分析。 该计划提供了分阶段的开发工作，将使 Sterling 博士做好准备 作为独立研究员或主要成员竞争 NIH 支持 一个多学科研究团队。