CORE--NEUROPHYSIOLOGY

核心--神经生理学

• 批准号: 6587615
• 负责人: LYNNE A FIEBER
• 金额: $ 7.35万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-22 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6587615
• 关键词: Aplysia; ammonia; aquatic organism; biomedical facility; electrophysiology; fish; fish poison; fresh water environment; marine toxins; neurophysiology; saltwater environment; single cell analysis; toxin metabolism; voltage /patch clamp; voltage gated channel

Description (provided by applicant): Electrophysiology is a technique that invites consideration of appropriate experiments from the Research Cores. The actions of aquatic toxins are often visible at the neurophysiological level. In the case of the Toxins Core, electrophysiology provides a sophisticated, but quite obvious, bioassay capability. Application of electrophysiological techniques to the Animal Models Core is more diverse in scope, but a unifying principle is that many of our animal models have a neurophysiological component. Electrophysiological applications range from basic studies of how the nervous systems of aquatic animals work to directed investigations on models of disease syndromes. The relevance of a Core to these animal models includes such long-exploited research programs as DNF and relatively new or emerging ones such as the toadfish hyperammonemia model. Additionally, a Core provides a bridge between toxin and Animal Models Research Cores, such as an attempt to study toxin effects on development in zebrafish larvae or the planned studies of toxin effects on hearing in sleeper gobies. Critical to these projects is the expertise of Core Leader Lynne Fieber, who can either execute the experiments or provide training for postdoctoral fellows who plan to dedicate concentrated effort to a particular project. The latter is not trivial as a Core service, because training to the neophyte electrophysiologist stage may take 6 months. But a Core is more than just single cell electrophysiology. A Core also provides Fura 2 apparatus and expertise, which is an interest of several of the investigators (e. g., Gawley via Berry). The proposed research on the toadfish hyperammonemia model will include new neurophysiological experiments requiring expansion of the core to include brain slice technology.

描述（由申请人提供）：电生理学是一种技术， 邀请研究核心考虑适当的实验。这 水生毒素的作用通常在神经生理学层面可见。 就毒素核心而言，电生理学提供了复杂的、 但相当明显的是，生物测定能力。电生理学的应用 动物模型核心的技术在范围上更加多样化，但统一的 原则是我们的许多动物模型都具有神经生理学特征 成分。电生理学的应用范围包括如何进行基础研究 水生动物的神经系统致力于定向研究 疾病综合症模型。核心与这些动物模型的相关性 包括诸如 DNF 等长期开发的研究项目以及相对较新或 新兴的模型，例如蟾蜍高氨血症模型。此外，还有一个核心 提供了毒素和动物模型研究核心之间的桥梁，例如 尝试研究毒素对斑马鱼幼虫或斑马鱼发育的影响 计划研究毒素对睡眠虾虎鱼听力的影响。 这些项目的关键在于核心领导者 Lynne Fieber 的专业知识，她 可以执行实验或为博士后提供培训 计划集中精力致力于特定项目的研究员。这 后者作为核心服务并非微不足道，因为对新手的培训 电生理学家阶段可能需要6个月。 但核心不仅仅是单细胞电生理学。 还有一个核心 提供 Fura 2 设备和专业知识，这引起了一些人的兴趣 调查人员（例如，Gawley via Berry）。拟议的研究 蟾蜍高氨血症模型将包括新的神经生理学实验 需要扩展核心以包括脑切片技术。