REGULATION OF THE MEIOTIC CELL CYCLE

减数分裂细胞周期的调节

• 批准号: 6520358
• 负责人: ANGELIKA B AMON
• 金额: $ 22.34万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6520358
• 关键词: Saccharomyces cerevisiae; chromosome movement; fungal genetics; fungal proteins; genetic recombination; meiosis; nucleoproteins; protein structure function

DESCRIPTION: During the mitotic cycle a single round of DNA replication is followed by a chromosome segregation phase, during which the duplicated genetic material is partitioned between the daughter cells. Meiosis is a specialized cell cycle in which a single DNA replication phase precedes two consecutive chromosome segregation phases. Meiosis-specific regulators of chromosome segregation are likely to act on the machinery common to mitotic and meiotic chromosome segregation to bring about the meiotic chromosome segregation program. A specialized ubiquitin-dependent proteolysis machinery, the APC (anaphase promoting complex), is a key regulator of chromosome segregation during mitosis. In Saccharomyces cerevisiae, the Pi has shown that the APC regulates meiotic recombination, which is critical for the first meiotic chromosome segregation phase. The protein Spo13 is a meiosis-specific regulator of chromosome segregation. The goals of this proposal are (1) to determine the role of the APC during meiotic recombination, (2) to determine how Spo13 controls meiotic chromosome segregation and (3) to identify new genes that regulate meiotic chromosome segregation. To characterize the role of the APC during meiotic recombination, genetic and molecular assays for recombination will be used to determine the step during recombination at which the APC is required. Biochemical and genetic approaches will then be used to identify APC target(s) whose destruction is required for the execution of meiotic recombination. Two approaches will be taken to identify meiosis-specific regulators of chromosome segregation. First, Spo13, which is known to regulate meiotic chromosome segregation will be studied to determine how it regulates this process and to identify its targets. Second, a genetic screen will be employed to identify new genes that regulate meiotic but not mitotic chromosome segregation.

描述：在有丝分裂周期中，一轮DNA复制为 然后是染色体分离阶段，在此期间重复的遗传 材料在子细胞之间分配。减数分裂是一种专业 单个DNA复制阶段在两个连续的细胞周期之前 染色体分离阶段。染色体的减数分裂特异性调节剂 种族隔离可能对有丝分裂和减数分裂的机械作用 染色体隔离以实现减数分裂染色体隔离 程序。一种专门的泛素依赖性蛋白水解机制，APC （后期促进复合物），是染色体隔离的关键调节剂 在有丝分裂期间。在酿酒酵母中，PI表明APC 调节减数分裂重组，这对于第一个减数分裂至关重要 染色体分离阶段。蛋白SPO13是减数分裂特异性调节剂 染色体隔离。 该提案的目标是（1）确定APC在 减数分裂重组，（2）确定SPO13如何控制减数分裂染色体 隔离和（3）鉴定调节减数分裂染色体的新基因 隔离。为了表征APC在减数分裂重组中的作用， 重组的遗传和分子测定将用于确定 在需要APC的重组过程中步骤。生化和遗传 然后将使用方法来识别其破坏是的APC目标 执行减数分裂重组所必需的。两种方法将是 用于鉴定染色体分离的减数分裂特异性调节剂。第一的， SPO13，已知会调节减数分裂染色体隔离的SPO13将是 研究以确定如何调节此过程并确定其目标。 其次，将采用遗传屏幕来识别调节的新基因 减数分裂，但不是有丝分裂的染色体分离。