EICOSANOIC ACIDS IN TRYPANOSOMA BRUCEI

布氏锥虫中的二十烷酸

• 批准号: 6510368
• 负责人: Lawrence Ruben
• 金额: $ 20.07万
• 依托单位: SOUTHERN METHODIST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-03-01 至 2004-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6510368
• 关键词: Trypanosoma brucei rhodesiense; biological signal transduction; calcium ion; cell death; eicosanoid metabolism; eicosanoids; endoplasmic reticulum; enzyme activity; fatty acid biosynthesis; laboratory mouse; life cycle; molecular cloning; phospholipase A2; protein purification; protein sequence

DESCRIPTION (Adapted from the Applicant's Abstract): This proposal is based on the hypothesis that African trypanosomes utilize a variety of signaling pathways to coordinate life cycle events. During the course of studies on the control of Ca+ currents in T. brucei, they discoved key components of an eicosanoic acid pathway. The present proposal will examine how trypanosomes regulate Ca+/eicosanoic acid signals.

描述（改编自申请人的摘要）：该提案基于 假设非洲锥虫利用多种信号传导 协调生命周期事件的途径。 在学习期间 通过控制 T. brucei 中的 Ca+ 电流，他们发现了 二十烷酸途径。 本提案将研究锥虫如何 调节 Ca+/二十烷酸信号。

项目成果

The gene family of EF-hand calcium-binding proteins from the flagellum of Trypanosoma brucei.

来自布氏锥虫鞭毛的 EF-hand 钙结合蛋白基因家族。

• DOI: 10.1042/bj3040833
• 发表时间: 1994
• 期刊: The Biochemical journal
• 作者: Wu,Y;Deford,J;Benjamin,R;Lee,MG;Ruben,L
• 通讯作者: Ruben,L

Trypanosoma brucei: the tumor promoter thapsigargin stimulates calcium release from an intracellular compartment in slender bloodstream forms.

布氏锥虫：肿瘤启动子毒胡萝卜素刺激细胞内细长血流形式的钙释放。

• DOI: 10.1016/0014-4894(92)90157-6
• 发表时间: 1992
• 期刊: Experimental parasitology
• 影响因子: 2.1
• 作者: Ruben,L;Akins,CD
• 通讯作者: Akins,CD

Phospholipase from Trypanosoma brucei releases arachidonic acid by sequential sn-1, sn-2 deacylation of phospholipids.

布氏锥虫的磷脂酶通过磷脂的连续 sn-1、sn-2 脱酰作用释放花生四烯酸。

• DOI: 10.1016/s0166-6851(01)00234-1
• 发表时间: 2001
• 期刊: Molecular and biochemical parasitology
• 影响因子: 1.5
• 作者: Ridgley,EL;Ruben,L
• 通讯作者: Ruben,L

Reactive oxygen species activate a Ca2+-dependent cell death pathway in the unicellular organism Trypanosoma brucei brucei.

活性氧物质激活单细胞生物布氏锥虫中的 Ca2 依赖性细胞死亡途径。

• 发表时间: 1999
• 期刊: The Biochemical journal
• 作者: Ridgley,EL;Xiong,ZH;Ruben,L
• 通讯作者: Ruben,L

Selective transfer of calcium from an acidic compartment to the mitochondrion of Trypanosoma brucei. Measurements with targeted aequorins.

钙从酸性区室选择性转移至布氏锥虫线粒体。

• DOI: 10.1074/jbc.272.49.31022
• 发表时间: 1997
• 期刊: The Journal of biological chemistry
• 作者: Xiong,ZH;Ridgley,EL;Enis,D;Olness,F;Ruben,L
• 通讯作者: Ruben,L